Team:Heidelberg/Notebook/Killing I/Cloning



Killing I - Cloning strategy

Our goal to clone a modified bacteriophage λ which can be transported by conjugation, allows the selection of infected cells through a chloamphenicol resistance and harbours GFP for visualization was designed in two strategies whereas strategy two builds on strategy one. A detailed description of the two strategies can be seen below (see 1.1 and 1.2). The used λ cI protein generator was synthesised by geneart and just transferred in a standard plasmid as it can be seen in 1.3.

phage cloning strategy one

HD phage1 klein.gif

phage cloning strategy two

HD phage2 klein.png

Bacteriophage λ cI cloning strategy

HD phage3.png