Imperial College/25 July 2008

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June
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[http://2008.igem.org/wiki/index.php?title=Imperial_College/9_June_2008&action=edit 9] [http://2008.igem.org/wiki/index.php?title=Imperial_College/10_June_2008&action=edit 10] [http://2008.igem.org/wiki/index.php?title=Imperial_College/11_June_2008&action=edit 11] [http://2008.igem.org/wiki/index.php?title=Imperial_College/12_June_2008&action=edit 12] [http://2008.igem.org/wiki/index.php?title=Imperial_College/13_June_2008&action=edit 13] [http://2008.igem.org/wiki/index.php?title=Imperial_College/14_June_2008&action=edit 14] [http://2008.igem.org/wiki/index.php?title=Imperial_College/15_June_2008&action=edit 15]
[http://2008.igem.org/wiki/index.php?title=Imperial_College/16_June_2008&action=edit 16] [http://2008.igem.org/wiki/index.php?title=Imperial_College/17_June_2008&action=edit 17] [http://2008.igem.org/wiki/index.php?title=Imperial_College/18_June_2008&action=edit 18] [http://2008.igem.org/wiki/index.php?title=Imperial_College/19_June_2008&action=edit 19] [http://2008.igem.org/wiki/index.php?title=Imperial_College/20_June_2008&action=edit 20] [http://2008.igem.org/wiki/index.php?title=Imperial_College/21_June_2008&action=edit 21] [http://2008.igem.org/wiki/index.php?title=Imperial_College/22_June_2008&action=edit 22]
[http://2008.igem.org/wiki/index.php?title=Imperial_College/23_June_2008&action=edit 23] [http://2008.igem.org/wiki/index.php?title=Imperial_College/24_June_2008&action=edit 24] [http://2008.igem.org/wiki/index.php?title=Imperial_College/25_June_2008&action=edit 25] [http://2008.igem.org/wiki/index.php?title=Imperial_College/26_June_2008&action=edit 26] [http://2008.igem.org/wiki/index.php?title=Imperial_College/27_June_2008&action=edit 27] [http://2008.igem.org/wiki/index.php?title=Imperial_College/28_June_2008&action=edit 28] [http://2008.igem.org/wiki/index.php?title=Imperial_College/29_June_2008&action=edit 29]
[http://2008.igem.org/wiki/index.php?title=Imperial_College/30_June_2008&action=edit 30]
July
MTWTFSS
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[http://2008.igem.org/Imperial_College/7_July_2008 7] [http://2008.igem.org/Imperial_College/8_July_2008 8] [http://2008.igem.org/Imperial_College/9_July_2008 9] [http://2008.igem.org/wiki/index.php?title=Imperial_College/10_July_2008&action=edit 10] [http://2008.igem.org/Imperial_College/11_July_2008 11] [http://2008.igem.org/wiki/index.php?title=Imperial_College/12_July_2008&action=edit 12] [http://2008.igem.org/wiki/index.php?title=Imperial_College/13_July_2008&action=edit 13]
[http://2008.igem.org/wiki/index.php?title=Imperial_College/14_July_2008&action=edit 14] [http://2008.igem.org/Imperial_College/15_July_2008 15] [http://2008.igem.org/Imperial_College/16_July_2008 16] [http://2008.igem.org/wiki/index.php?title=Imperial_College/17_July_2008&action=edit 17] [http://2008.igem.org/Imperial_College/18_July_2008 18] [http://2008.igem.org/wiki/index.php?title=Imperial_College/19_July_2008&action=edit 19] [http://2008.igem.org/wiki/index.php?title=Imperial_College/20_July_2008&action=edit 20]
[http://2008.igem.org/Imperial_College/21_July_2008 21] [http://2008.igem.org/Imperial_College/22_July_2008 22] [http://2008.igem.org/Imperial_College/23_July_2008 23] [http://2008.igem.org/Imperial_College/24_July_2008 24] [http://2008.igem.org/Imperial_College/25_July_2008 25] [http://2008.igem.org/wiki/index.php?title=Imperial_College/26_July_2008&action=edit 26] [http://2008.igem.org/wiki/index.php?title=Imperial_College/27_July_2008&action=edit 27]
[http://2008.igem.org/Imperial_College/28_July_2008 28] [http://2008.igem.org/Imperial_College/29_July_2008 29] [http://2008.igem.org/Imperial_College/30_July_2008 30] [http://2008.igem.org/wiki/index.php?title=Imperial_College/31_July_2008&action=edit 31]
August
MTWTFSS
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[http://2008.igem.org/Imperial_College/4_August_2008 4] [http://2008.igem.org/Imperial_College/5_August_2008 5] [http://2008.igem.org/wiki/index.php?title=Imperial_College/6_August_2008&action=edit 6] [http://2008.igem.org/Imperial_College/7_August_2008 7] [http://2008.igem.org/Imperial_College/8_August_2008 8] [http://2008.igem.org/wiki/index.php?title=Imperial_College/9_August_2008&action=edit 9] [http://2008.igem.org/Imperial_College/10_August_2008 10]
[http://2008.igem.org/Imperial_College/11_August_2008 11] [http://2008.igem.org/Imperial_College/12_August_2008 12] [http://2008.igem.org/Imperial_College/13_August_2008 13] [http://2008.igem.org/Imperial_College/14_August_2008 14] [http://2008.igem.org/Imperial_College/15_August_2008 15] [http://2008.igem.org/Imperial_College/16_August_2008 16] [http://2008.igem.org/Imperial_College/17_August_2008 17]
[http://2008.igem.org/Imperial_College/18_August_2008 18] [http://2008.igem.org/Imperial_College/19_August_2008 19] [http://2008.igem.org/Imperial_College/20_August_2008 20] [http://2008.igem.org/Imperial_College/21_August_2008 21] [http://2008.igem.org/Imperial_College/22_August_2008 22] [http://2008.igem.org/wiki/index.php?title=Imperial_College/23_August_2008&action=edit 23] [http://2008.igem.org/Imperial_College/24_August_2008 24]
[http://2008.igem.org/Imperial_College/25_August_2008 25] [http://2008.igem.org/Imperial_College/26_August_2008 26] [http://2008.igem.org/Imperial_College/27_August_2008 27] [http://2008.igem.org/Imperial_College/28_August_2008 28] [http://2008.igem.org/Imperial_College/29_August_2008 29] [http://2008.igem.org/wiki/index.php?title=Imperial_College/30_August_2008&action=edit 30] [http://2008.igem.org/Imperial_College/31_August_2008 31]

25 July 2008

Debriefing

Presentation was given summarizing the project so far (upload presentation). Several key issues were raised during the presentation:

  • Need to order sequence and reagents asap,
  • On Monday 11.30 the DNA illuminator will have a demo and on Tuesday the fluorometer will.
  • Need to contact the imaging center to begin training on the wide field microscope,
  • QQ needs to contact Tony Cass about his experience expressing elastin protein in bacteria,
  • Group clearly needs to be restructured, need to think about how to organize ourselves from now on. Going to discuss on Monday how we can move forward.
  • Feedback about our design so far:
    • Integration sites - Is there we can design them to allow assembly in B.subtilis, e.g. if we want to insert another biobrick to the beginning or the end of a construct, or will we have to assemble the constucts in E.coli
    • Genetic Circuit - Questions over trying to suppress the natural EspE by overexpressing SinR, this could have diverse effects to the cell. Also do we want to have a memory switch, so that when we induce the expression of biomaterials with blue light, then we will continue to produce biomaterials rather than requiring constant or frequent exposures to potentially harmful light.
    • Think modular and think about the different constructs that we are going to add to the registry!