Team:Hawaii/Notebook/2008-06-19

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Things we did today

Wetlab work

Alkaline Lysis Miniprep

Krystle & Margaret
  • Plasmid prep was performed according to the experimental protocol
  • Plasmid prep was performed with:
BioBricks
part number antibx media strength source organism
E0040 (GFP) amp100 DH5&alpha
J04430 (lac+GFP) DH5&alpha
B0034 (RBS) GP's super competent cells
B0024 (TT) DH5&alpha


Tool Plasmids
type antibx media strength source organism
pUC18 amp100 DH5&alpha
pBLUSCRIPT
RP1 kan50 E. coli

Cryo-Stocks

Krystle
  • Made cryo-stocks of pBLUSCRIPT, pUC18, and B0024.

Run Gel for pRL1383a plasmid Prep

Norman
  • See experiment for details.

Drylab Work

Primer Discussion

Krystle, Norman, & Margaret
  • Discussed the format of primers
  • worked on revising primers


Discussion

Quote of the Day

Note to self: if I have weird cancers in strange places, its probably because I was playing with these carcinogens. - KS


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