20 May 2008

From 2008.igem.org

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Revision as of 20:22, 20 May 2008

To Do

Today we are researching PIF3 degredation as it might occur in Ecoli

Writing the new fundraising letters

Finishing construction of our biobricks

Colony PCR,Gel extraction, and streaking positive colonies and setting up O/N cultures of last years project.

Chris
- Ran the thiolase sequencing that I did last week up to the MBSU. We should get the results back tomorrow.

- Finished up the BioBricks we've worked on so far: PIF3 and the Phytochromobilin/Heme Oxygenase Operon. The HIS-tags have been added to all three genes and they have been optimized for expression in E.coli (strain K12, specificly, though we are going to be using XL10. The optimization site I mentioned in the May 16th notes did not have the XL10 strain listed so I went with K12. I don't anticipate this being a problem). The Phytochromobilin/Heme Oxygenase operon looks like this:

The black squares at either end are the standard BioBrick suffix and prefix. The blue represents a terminator sequence (part BBa_B1001) and the red diamonds mark where we put in the RBS (part BBa_B0034).

-I present to thee: FLUFFY, THE OFFICIAL UofA 2008 iGEM TEAM LOLCAT

Lab Tip of The Day

If you can't log into a MAC computer DONT put you CD in it as you will not be able to retrive it

@@ Line 19: / Line 19: @@
 -I present to thee: FLUFFY, THE OFFICIAL UofA 2008 iGEM TEAM LOLCAT<br>
 [[Image:lolcat_biobrick.jpg]]
-'''Lab Tip Of the Day''': When inserting a DVD into a MAC computer, please ensure that you have access to said computer or else you will be unable to retrieve your DVD. Please remember to carry a 2008 Official iGEM Pointed DVD Removal Device (aka a stick) with you at all times to aid in DVD removal in the event of catastrophic DVD loss.
 ==Lab Tip of The Day==
 '''If you can't log into a MAC computer DONT put you CD in it as you will not be able to retrive it'''