From 2008.igem.org
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| - | ==Volunteers Scheduled for Today==
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| - | 5-8 AS<br>
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| - | 6-8 DL<br>
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| - | Things to do:<br>
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| - | Mini-preps of O/N's<br>
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| - | Sequence gel purified fragments from yesterday<br>
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| - | Transform I0500<br>
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| - | Digest J61003 with Xba/Pst to insert ORF's<br>
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| - | Digest minipreps with Xba/Pst to insert into J61003<br>
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| - | Run gel of digests and excise correct fragments<br>
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| - | Ligate vector/inserts<br>
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| - | Transform ligation reactions<br>
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| - | Lab clean up<br>
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| - | Checked all O/N's set up by SZ and WX - all grew except 023 WX. Will set up again tonight.<br>
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| - | Followed standard Qiagen miniprep protocol for all o/n's.<br>
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| - | ==The Good and Bad news==
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| - | <u>'''The Good News'''</U><br>
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| - | Our system will work in E.coli<br>
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| - | <u>'''The Bad News'''</U><br>
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| - | In 2004 Texas submitted the cyanbacterial equivalent of our system, so... Our biobricks would be unique but they would do the exact same thing.<br>
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| - | <u>'''The Good News'''</U><br>
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| - | I looked up a very promising pathway that detects blue light instead<br>
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| - | <u>'''The Bad News'''</U><br>
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| - | I can only find a summary of the paper... Here is the link to it
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| - | http://www.arabidopsis.org/servlets/TairObject?type=publication&id=501724582
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Latest revision as of 19:20, 22 May 2008
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