From 2008.igem.org
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lab work (SD):
Ligation of TA2, TA3, TA6 and TA7 into vector K102031.
Transformed XL1-B cells with the ligations.
lab work (JD):
Annealed TAIn_2, TAIn_3, TAIn_6 and TAIn_7
Nucleotide Removal
Restriction Digest (2hrs) with XhoI + HindIII
Ran on gel 2%, 80V for 2 hours
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