Alberta NINT/11 July 2008

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lab work (SD):
lab work (SD):
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             Lac Z Activity Assay was unsuccessful - perhaps not enough cells were used?
             Lac Z Activity Assay was unsuccessful - perhaps not enough cells were used?
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[[Alberta_NINT/14_July_2008 | Next entry >]]
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lab work (JD):
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            Carried out Day 2 protocol for Activity Assay
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            Like stated above, was not successful.
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            Collected quantitative data of restriction fragments for ligation
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            Ligated K102003+pSB4A5=K102031 and K102006+pSB4A5=K102021
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            Transformed XL1-B Cells
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            Plated cells on LB + Amp50

Latest revision as of 22:59, 11 October 2008

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lab work (SD):

            Measured cell count using spectrophotometer of 10/07/08 overnights.
            Lac Z Activity Assay was unsuccessful - perhaps not enough cells were used?

lab work (JD):

            Carried out Day 2 protocol for Activity Assay
            Like stated above, was not successful.
            Collected quantitative data of restriction fragments for ligation
            Ligated K102003+pSB4A5=K102031 and K102006+pSB4A5=K102021
            Transformed XL1-B Cells 
            Plated cells on LB + Amp50