"
Alberta NINT/20 August 2008
From 2008.igem.org
(Difference between revisions)
m |
|||
| Line 1: | Line 1: | ||
[[Team:Alberta_NINT/Notebook | < Back to notebook]] | [[Team:Alberta_NINT/Notebook | < Back to notebook]] | ||
| - | [[Alberta_NINT/19_August_2008 | < Previous entry]] | + | [[Alberta_NINT/19_August_2008 | < Previous entry]] | [[Alberta_NINT/21_August_2008 | Next entry >]] |
| + | |||
lab work (SD): | lab work (SD): | ||
| Line 15: | Line 16: | ||
Miniprep of O/N of cotransformed cells | Miniprep of O/N of cotransformed cells | ||
Day 2 of LacZ Assay | Day 2 of LacZ Assay | ||
| - | |||
| - | |||
Latest revision as of 23:10, 11 October 2008
< Previous entry | Next entry >
lab work (SD):
Digested TA2+(PCR) with BamHI+NcoI. Incubated at 37 C for 1 hr.
Ran TA2+(PCR) (10ul) and TA2+(PCR)/B+N (10ul) on 2% agarose gel 110 V 60 min
Used nucleotide removal purification kit to purify 10ul of TA2+(PCR)/B+N.
4.4 ng/ul 1.96 A260/280 67 bp = 99.499 fmol/ul
The gel did not have a band for the 67 bp TA2+(PCR)/B+N. This may be because the concentration is
too low.
lab work (JD):
Miniprep of O/N of cotransformed cells
Day 2 of LacZ Assay
