Alberta NINT/23 July 2008
From 2008.igem.org
(Difference between revisions)
(New page: < Back to notebook < Previous entry lab work (SD): Results from 22/07/08 transformation: ...) |
m |
||
Line 1: | Line 1: | ||
[[Team:Alberta_NINT/Notebook | < Back to notebook]] | [[Team:Alberta_NINT/Notebook | < Back to notebook]] | ||
- | [[Alberta_NINT/22_July_2008 | < Previous entry]] | + | [[Alberta_NINT/22_July_2008 | < Previous entry]] | [[Alberta_NINT/24_July_2008 | Next entry >]] |
+ | |||
lab work (SD): | lab work (SD): | ||
Line 14: | Line 15: | ||
Isolated DNA from overnights of K102011, K102034, K102035, and K102036, using 3 ml to pellet cells | Isolated DNA from overnights of K102011, K102034, K102035, and K102036, using 3 ml to pellet cells | ||
using QIAprep spin miniprep standard protocol (to obtain more DNA for these constructs). | using QIAprep spin miniprep standard protocol (to obtain more DNA for these constructs). | ||
- | |||
- |
Latest revision as of 23:02, 11 October 2008
< Previous entry | Next entry >
lab work (SD):
Results from 22/07/08 transformation: LB+amp50 LB+kan35 LB+amp50+kan35 WM >100 >100 1 SD >100 >100 16 Proceeded with B-Gal Activity Assay when cell cultures grown in the 96-well plate from 21/07/08 showed sufficient cell growth after two days at 37 C. Prepared media (LB+antibiotics+IPTG+glucose/arabinose) and inoculated 1 ml of media with 20 ul of cell cultures from the 21/07/08 96-well plate. Incubated at 37 C overnight. Isolated DNA from overnights of K102011, K102034, K102035, and K102036, using 3 ml to pellet cells using QIAprep spin miniprep standard protocol (to obtain more DNA for these constructs).