Alberta NINT/23 June 2008

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(Difference between revisions)
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lab work (SD):
lab work (SD):
             Transformed XL1-B cells with K102002/B+N ligated to TA0/B+N (K102008).
             Transformed XL1-B cells with K102002/B+N ligated to TA0/B+N (K102008).
 +
 +
lab work (JD):
 +
              Quantitated K102005/B+N             
 +
              Ligated K102005/B+N with the newly annealed TA0/B+N=K102007
 +
              Transformed XL1-B cells with K102007
 +
              Plated cells on LB+Amp50
 +
              From sequencing K102003.4 becomes official K102003.
[[Alberta_NINT/24_June_2008 | Next entry >]]
[[Alberta_NINT/24_June_2008 | Next entry >]]

Revision as of 21:00, 26 June 2008

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lab work (SD):

            Transformed XL1-B cells with K102002/B+N ligated to TA0/B+N (K102008).

lab work (JD):

             Quantitated K102005/B+N              
             Ligated K102005/B+N with the newly annealed TA0/B+N=K102007
             Transformed XL1-B cells with K102007
             Plated cells on LB+Amp50
             From sequencing K102003.4 becomes official K102003.

Next entry >