Alberta NINT/23 June 2008
From 2008.igem.org
(Difference between revisions)
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lab work (SD): | lab work (SD): | ||
Transformed XL1-B cells with K102002/B+N ligated to TA0/B+N (K102008). | Transformed XL1-B cells with K102002/B+N ligated to TA0/B+N (K102008). | ||
+ | |||
+ | lab work (JD): | ||
+ | Quantitated K102005/B+N | ||
+ | Ligated K102005/B+N with the newly annealed TA0/B+N=K102007 | ||
+ | Transformed XL1-B cells with K102007 | ||
+ | Plated cells on LB+Amp50 | ||
+ | From sequencing K102003.4 becomes official K102003. | ||
[[Alberta_NINT/24_June_2008 | Next entry >]] | [[Alberta_NINT/24_June_2008 | Next entry >]] |
Revision as of 21:00, 26 June 2008
lab work (SD):
Transformed XL1-B cells with K102002/B+N ligated to TA0/B+N (K102008).
lab work (JD):
Quantitated K102005/B+N Ligated K102005/B+N with the newly annealed TA0/B+N=K102007 Transformed XL1-B cells with K102007 Plated cells on LB+Amp50 From sequencing K102003.4 becomes official K102003.