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Alberta NINT/27 May 2008
From 2008.igem.org
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lab work (SD): DNA digest of K102000 with SpeI + PstI. Incubated at 37 C for 1 hour. Run on 2% agarose gel. ~3 kbp fragment expected. Ligated K102000 with E0433 and K102000 with null_output. | lab work (SD): DNA digest of K102000 with SpeI + PstI. Incubated at 37 C for 1 hour. Run on 2% agarose gel. ~3 kbp fragment expected. Ligated K102000 with E0433 and K102000 with null_output. | ||
| + | lab work (JD): Recovered Part BBA_R011 from iGEM package, transformed XL1-B cells and plated on LB+Amp100 plates. Created 2 O/Ns as well. | ||
Revision as of 22:17, 30 May 2008
lab work (SD): DNA digest of K102000 with SpeI + PstI. Incubated at 37 C for 1 hour. Run on 2% agarose gel. ~3 kbp fragment expected. Ligated K102000 with E0433 and K102000 with null_output. lab work (JD): Recovered Part BBA_R011 from iGEM package, transformed XL1-B cells and plated on LB+Amp100 plates. Created 2 O/Ns as well.
