Alberta NINT/3 June 2008

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[[Team:Alberta_NINT/Notebook | < Back to notebook]]
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[[Alberta_NINT/2_June_2008 | < Previous entry]] | [[Alberta_NINT/4_June_2008 | Next entry >]]
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lab work (SD):  
lab work (SD):  
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             Used QIAquick nucleotide removal standard protocol to purify T/A 0.   
             Used QIAquick nucleotide removal standard protocol to purify T/A 0.   
             Ligated T/A 0 into K102002/B+N and K102004/B+N.
             Ligated T/A 0 into K102002/B+N and K102004/B+N.
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[[Image:NINT_SD_p25.jpg|750 px]]
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lab work (WM):
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          #colonies K102011:    48          negative ctl:  0
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          Picked 4 colonies for O/Ns

Latest revision as of 22:16, 11 October 2008

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lab work (SD):

           Ran K102002/B+N and K102004/B+N on 2% agarose gel to purify.  
           Used QIAquick nucleotide removal standard protocol to purify T/A 0.  
           Ligated T/A 0 into K102002/B+N and K102004/B+N.

NINT SD p25.jpg

lab work (WM):

          #colonies K102011:    48           negative ctl:  0
          Picked 4 colonies for O/Ns