Brown: Team Resistance/25 September 2008

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(New page: * Growth seen in 3:1 and 6:1 ligations! * Ligation cultures miniprepped. * 3:1 Restriction Digest (X2) # 1 ul EcoRI # 1 ul HindIII # 5 ul Buffer E # .5 ul BSA # 10 ul DNA # 32.5 ul steril...)
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== 25 September 2008 ==
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* Growth seen in 3:1 and 6:1 ligations!
* Growth seen in 3:1 and 6:1 ligations!
* Ligation cultures miniprepped.
* Ligation cultures miniprepped.

Revision as of 01:25, 21 October 2008


Toxipop lab notebook.png


25 September 2008

  • Growth seen in 3:1 and 6:1 ligations!
  • Ligation cultures miniprepped.
  • 3:1 Restriction Digest (X2)
  1. 1 ul EcoRI
  2. 1 ul HindIII
  3. 5 ul Buffer E
  4. .5 ul BSA
  5. 10 ul DNA
  6. 32.5 ul sterilized water
  • 6:1 Restriction Digest (X2)
  1. 1 ul EcoRI
  2. 1 ul HindIII
  3. 5 ul Buffer E
  4. .5 ul BSA
  5. 9 ul DNA
  6. 33.5 ul sterilized water
Retrieved from "http://2008.igem.org/Brown:_Team_Resistance/25_September_2008"