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Edinburgh/19 July 2008
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| - | ==== Saturday 19 July 08 | + | <html> |
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| + | {| align="left" | ||
| + | |- | ||
| + | |align="left" width="150pt"|{{#calendar: title=Edinburgh |year=2008 | month=06}} | ||
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| + | |align="left" width="150pt"|{{#calendar: title=Edinburgh |year=2008 | month=07}} | ||
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| + | |align="left" width="150pt"|{{#calendar: title=Edinburgh |year=2008 | month=08}} | ||
| + | |} | ||
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| + | :::: '''[[Edinburgh/18_July_2008|< Previous Entry]]''' | ||
| + | |||
| + | == Week 5 == | ||
| + | === Saturday 19 July 08 === | ||
* Minipreps '''M43 to M47''' of possible ''glgC'' double mutants (both EcoRI sites gone), and '''M48''' of the only white colony from the pSB1A2+rbs+''dxs'' transformation. '''Gel 18''' shows EcoRI/PstI digests of 3μl. M43 to M47 all look about right: send one for sequencing. M48 is ambiguous, could be incomplete deigestion, need to repeat. (CF) | * Minipreps '''M43 to M47''' of possible ''glgC'' double mutants (both EcoRI sites gone), and '''M48''' of the only white colony from the pSB1A2+rbs+''dxs'' transformation. '''Gel 18''' shows EcoRI/PstI digests of 3μl. M43 to M47 all look about right: send one for sequencing. M48 is ambiguous, could be incomplete deigestion, need to repeat. (CF) | ||
| - | * PCR '''P24 and P25''', repeat PCR for ''cenA'' and ''cex'' (the two shorter genes) using Pfu polymerase instead of KOD, adding 5% glycerol - this worked well for high-GC genes from ''Saccharothrix espanaensis'' last year. (CF) | + | * PCR '''P24 and P25''', repeat PCR for ''cenA'' and ''cex'' (the two shorter genes) using Pfu polymerase instead of KOD, adding 5% glycerol - this worked well for high-GC genes from ''Saccharothrix espanaensis'' last year. (CF)<br /> |
| + | <br /> | ||
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| + | :::: '''[[Edinburgh/20_July_2008|Next Entry >]]''' | ||
Revision as of 16:17, 21 August 2008
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Week 5
Saturday 19 July 08
- Minipreps M43 to M47 of possible glgC double mutants (both EcoRI sites gone), and M48 of the only white colony from the pSB1A2+rbs+dxs transformation. Gel 18 shows EcoRI/PstI digests of 3μl. M43 to M47 all look about right: send one for sequencing. M48 is ambiguous, could be incomplete deigestion, need to repeat. (CF)
- PCR P24 and P25, repeat PCR for cenA and cex (the two shorter genes) using Pfu polymerase instead of KOD, adding 5% glycerol - this worked well for high-GC genes from Saccharothrix espanaensis last year. (CF)
