Edinburgh/27 July 2008

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=== Sunday 27 July 08 ===
=== Sunday 27 July 08 ===
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* Result of transformations: no growth for L19 (pSB1A2+rbs+''dxs''), L20 (pSB1A2+rbs+''appY'') or L21 (pSB1A2+rbs+''crtB''). For L22 (pSB1A2+rbs+''crtI'') there are some blue and white colonies. For L23 (pSB1A2+pZntA) there are many blue colonies but only a few white ones, suggesting that the SacI/SpeI double digest is less efficient than the XbaI/PstI which was (I hope) used for the others. For L24 (pSB1A2+''crtE'') there are many white colonies and very few blue - this is as expected, since in this case the vector and insert bands were purified from a gel. (CF)<br />
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* Result of transformations: no growth for L19 (pSB1A2+rbs+''dxs''), L20 (pSB1A2+rbs+''appY'') or L21 (pSB1A2+rbs+''crtB''). For L22 (pSB1A2+rbs+''crtI'') there are some blue and white colonies. For L23 (pSB1A2+''P<sub>zntA</sub>'') there are many blue colonies but only a few white ones, suggesting that the SacI/SpeI double digest is less efficient than the XbaI/PstI which was (I hope) used for the others. For L24 (pSB1A2+''crtE'') there are many white colonies and very few blue - this is as expected, since in this case the vector and insert bands were purified from a gel. (CF)<br />
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Latest revision as of 20:37, 29 October 2008

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Week 6

Sunday 27 July 08

  • Result of transformations: no growth for L19 (pSB1A2+rbs+dxs), L20 (pSB1A2+rbs+appY) or L21 (pSB1A2+rbs+crtB). For L22 (pSB1A2+rbs+crtI) there are some blue and white colonies. For L23 (pSB1A2+PzntA) there are many blue colonies but only a few white ones, suggesting that the SacI/SpeI double digest is less efficient than the XbaI/PstI which was (I hope) used for the others. For L24 (pSB1A2+crtE) there are many white colonies and very few blue - this is as expected, since in this case the vector and insert bands were purified from a gel. (CF)


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