Edinburgh/28 August 2008

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Week 11

Thursday 28 August 08

  • 18 subs made from plate 126 (pSB1A2+cenA) to plate 164. (AH)
  • Sequencing results received for M160 indicating that it is pSB1A2+dxs+lims (appY has not been added). On gel 62 and 65 M160 produced bands migrating more slowly than expected for pSB1A2+dxs+lims+appY, and M161 and M162 migrated more slowly still. Bearing in mind that M160 came out as being just pSB1A2+dxs+lims it might be worth sequencing M161 or M162. (AH)
  • Competent cells transformed with pSB1A2-CHU2268 (beta glucosidase, from P90) and plated onto Plates 165 (100µl) and 166 (900 µl). (AM)
  • ISA1/ZM1 (from P88) and ISA2/ZM2 (from P89) ligated to Babel1 and SOB2. (AM)
  • Ligation of appY into CrtBI, L57. (YAN)
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