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Edinburgh/5 September 2008
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=== Friday 5 September 08 === | === Friday 5 September 08 === | ||
| - | * '''P95:''' ''cex'' mutagenesis with M151 as template. Annealing temp 58 degrees, extension time 68 seconds. (Note: there was another microtube in the PCR product box, labelled as " | + | * '''P95:''' ''cex'' mutagenesis with M151 as template. Annealing temp 58 degrees, extension time 68 seconds. (Note: there was another microtube in the PCR product box, labelled as "cex mut", and I did not know who did it. So, I repeated this mutagenesis again, PCR products labelled as P95) (YAN). |
| - | * '''L62:''' ligation of dxs+crtE (digested out of M155) and crtBI (digested out of M110). Note: | + | * '''L62:''' ligation of ''dxs+crtE'' (digested out of M155) and ''crtBI'' (digested out of M110). Note: ''crtBI'' as vector, cut with EcoRI and XbaI; ''dxs+crtE'' as insert, cut with EcoRI and SpeI (YAN) |
| - | * | + | * Transformation of L58 to L61, followed by sub-cloning and overnight culturing (C.F) |
Latest revision as of 22:19, 29 October 2008
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Week 12
Friday 5 September 08
- P95: cex mutagenesis with M151 as template. Annealing temp 58 degrees, extension time 68 seconds. (Note: there was another microtube in the PCR product box, labelled as "cex mut", and I did not know who did it. So, I repeated this mutagenesis again, PCR products labelled as P95) (YAN).
- L62: ligation of dxs+crtE (digested out of M155) and crtBI (digested out of M110). Note: crtBI as vector, cut with EcoRI and XbaI; dxs+crtE as insert, cut with EcoRI and SpeI (YAN)
- Transformation of L58 to L61, followed by sub-cloning and overnight culturing (C.F)
