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Edinburgh/9 September 2008
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* Minipreps '''M198 to M209''': | * Minipreps '''M198 to M209''': | ||
| - | ** '''M198 to M200:''' L58 = self-ligation of P91 (SOB2- | + | ** '''M198 to M200:''' L58 = self-ligation of P91 (SOB2-''SU1''). M199 and M200 were missed out by accident. |
| - | ** '''M201 to M203:''' L59 = self-ligation of P92 (SOB2-rbs- | + | ** '''M201 to M203:''' L59 = self-ligation of P92 (SOB2-rbs-''SU1''). |
| - | ** '''M204 to M206:''' L60 = self-ligation of P93 (SOB2- | + | ** '''M204 to M206:''' L60 = self-ligation of P93 (SOB2-''ISO2''). |
| - | ** '''M207 to M209:''' L61 = self-ligation of P94 (SOB2-rbs- | + | ** '''M207 to M209:''' L61 = self-ligation of P94 (SOB2-rbs-''ISO2''). (Yan) |
** M198, M201 to 209 subsequently digested with EcoRI and PstI. (AM) | ** M198, M201 to 209 subsequently digested with EcoRI and PstI. (AM) | ||
| - | * Re-ran M182~187 (pSB1A2+cenA) on '''Gel 72''' and confirmed failure of pSB1A2+cenA ligation. (AM) | + | * Re-ran M182~187 (pSB1A2+''cenA'') on '''Gel 72''' and confirmed failure of pSB1A2+''cenA'' ligation. (AM) |
| - | * Ran P95 and 'cex mut' (both cex mutagenic PCR) on '''Gel 73'''. Both failed. (AM) | + | * Ran P95 and 'cex mut' (both ''cex'' mutagenic PCR) on '''Gel 73'''. Both failed. (AM) |
* Ligations '''L63 to L64:''' | * Ligations '''L63 to L64:''' | ||
| - | ** '''L63:''' P90 (CHU2268 bglX) to Edinbrick 1. | + | ** '''L63:''' P90 (CHU2268 ''bglX'') to Edinbrick 1. |
| - | ** '''L64:''' P65 (cenA) to Edinbrick 1. (AM) | + | ** '''L64:''' P65 (''cenA'') to Edinbrick 1. (AM) |
| - | * Submitted M161 and M162 (pSB1A2+dxs+lims+appY) for sequencing using reverse primer only. (AM) | + | * Submitted M161 and M162 (pSB1A2+''dxs+lims+appY'') for sequencing using reverse primer only. (AM) |
Latest revision as of 22:21, 29 October 2008
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Week 13
Tuesday 9 September 08
- Minipreps M198 to M209:
- M198 to M200: L58 = self-ligation of P91 (SOB2-SU1). M199 and M200 were missed out by accident.
- M201 to M203: L59 = self-ligation of P92 (SOB2-rbs-SU1).
- M204 to M206: L60 = self-ligation of P93 (SOB2-ISO2).
- M207 to M209: L61 = self-ligation of P94 (SOB2-rbs-ISO2). (Yan)
- M198, M201 to 209 subsequently digested with EcoRI and PstI. (AM)
- Re-ran M182~187 (pSB1A2+cenA) on Gel 72 and confirmed failure of pSB1A2+cenA ligation. (AM)
- Ran P95 and 'cex mut' (both cex mutagenic PCR) on Gel 73. Both failed. (AM)
- Ligations L63 to L64:
- L63: P90 (CHU2268 bglX) to Edinbrick 1.
- L64: P65 (cenA) to Edinbrick 1. (AM)
- Submitted M161 and M162 (pSB1A2+dxs+lims+appY) for sequencing using reverse primer only. (AM)
