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Edinburgh/Week 15
From 2008.igem.org
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** M233 submitted for sequencing. (CF) | ** M233 submitted for sequencing. (CF) | ||
| - | * Candidate clones for ''cenA'' ('''M237, | + | * Candidate clones for ''cenA'' ('''M237, 238''') obtained. |
** These seem to possess the expected internal SalI sites. | ** These seem to possess the expected internal SalI sites. | ||
** One of these has been submitted for sequencing. (CF) | ** One of these has been submitted for sequencing. (CF) | ||
| + | |||
| + | * Candidate clones for ''bglX'' ('''M240, 247''') obtained. | ||
| + | ** Submitted for sequencing but not so confident about these: the ratio of white to blue colonies was very low. There might be a problem with the restriction site in one of the primers. | ||
* rbs added to ''glgC'' and ''glgC16''; cloned as '''M243''' and '''M245'''. Both submitted for sequencing. (CF) | * rbs added to ''glgC'' and ''glgC16''; cloned as '''M243''' and '''M245'''. Both submitted for sequencing. (CF) | ||
Revision as of 19:15, 6 October 2008
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Week 15
- Clones for ISA1/zm1 (M229) and ISA2/zm2 (M230, 231, 232) obtained.
- These have the internal EcoRI as expected so they are probably the right genes but should be sequenced to confirm that the ends are intact.
- M229 (ISA1) and M231 (ISA2) submitted for sequencing. (CF)
- Clones for cex mutant (M233, 234, 235) obtained.
- These clearly do not have the internal PstI site.
- M233 submitted for sequencing. (CF)
- Candidate clones for cenA (M237, 238) obtained.
- These seem to possess the expected internal SalI sites.
- One of these has been submitted for sequencing. (CF)
- Candidate clones for bglX (M240, 247) obtained.
- Submitted for sequencing but not so confident about these: the ratio of white to blue colonies was very low. There might be a problem with the restriction site in one of the primers.
- rbs added to glgC and glgC16; cloned as M243 and M245. Both submitted for sequencing. (CF)
- lac promoter (J33207) added to dxs+lims+appY Biobrick.
- Does not smell of lemons but E. coli has a strong smell so this might not be significant.
- Arranged for GC to be done to test for limonene production. (CF)
- Ligation and transformation to add crtBI upstream of dxs+crtE. Minipreps to be done.
- None of the patches are pink, but then, there is no promoter. (CF)
- C. fimi genomic DNA submitted for sequencing. (CF)
