Experiment

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Experiment results
     
 

Synthetic convertible ecosystem

EG cells received 3OC6HSL signal molecular and expressed ampicillin resistance protein
 
ER cells received C4HSL signal molecular and expressed kanamycin resistance protein
The growth curve and the expression of the GFP of the EG cells in different condition
The growth curve and the expression of the GFP of the ER cells in different condition
ER overwhelms EG in population at the existence of antibiotics
 
Antibiotic changes detected by LC-MS/MS
 
Both ampicillin and kanamycin decrease with the increase of population density due to gradual decomposition of antibiotics. Most of antibiotics were decomposed at the lag phase and decreased very quickly to a low level at the beginning of the exponential phase.
Increase of initial population density enhances system resistance to antibiotics
EG= 3.33×106/ml
ER= 1×106/ml
 
EG= 5×106/ml
ER= 1×106/ml
 
EG= 1×107/ml
ER= 1×106/ml

 

AMP=4mg/ml
KAN=1mg/ml
 
AMP=8mg/ml
KAN=1mg/ml
 
AMP=8mg/ml
KAN=1.6mg/ml
simulation
 
 
Influence of antibiotics on population dynamics
EG= 1×107/ml
 
ER= 1×106/ml
 
EG= 5×106/ml
ER= 1×106/ml
simulation