Making Overnight Cultures from Agar Colonies

From 2008.igem.org

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(New page: * Pipette 10mL LB into red-capped culture tubes or conical shake flasks. * Using an autoclaved toothpick, gently touch the point to a single colony. * Drop the toothpick point down into th...)
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* Drop the toothpick point down into the LB.
* Drop the toothpick point down into the LB.
* Incubate overnight at 37°C whilst shaking.
* Incubate overnight at 37°C whilst shaking.
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'''Back to [[Team:Newcastle University/Protocols]]'''
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'''Back to [[Team:Newcastle University/Notebook]]'''

Revision as of 10:51, 18 September 2008

  • Pipette 10mL LB into red-capped culture tubes or conical shake flasks.
  • Using an autoclaved toothpick, gently touch the point to a single colony.
  • Drop the toothpick point down into the LB.
  • Incubate overnight at 37°C whilst shaking.


Back to Team:Newcastle University/Protocols

Back to Team:Newcastle University/Notebook