Minnesota/25 June 2008

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|1. '''Steak LB + antibiotic plate:'''  
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|1. '''Streak LB + antibiotic plate:'''  
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|a. From colonies whose plasmids we’ve sequenced, pick same colony and streak plates.Plates 6-14 were placed in the incubator @ 37C to regrow picked colonies for approximately 3 hours.
|a. From colonies whose plasmids we’ve sequenced, pick same colony and streak plates.Plates 6-14 were placed in the incubator @ 37C to regrow picked colonies for approximately 3 hours.
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|b. New LB plates with ampicillin were streaked with cells from the picked colonies for Biobricks 6-14. For each, a sterile pipette tip was dipped in the colony, and then used to streak a region of the new plate. A second sterile pipette tip was used to streak out of this region into an unstreaked section. Streaked plates were placed in incubator @ 37C to grow.
|b. New LB plates with ampicillin were streaked with cells from the picked colonies for Biobricks 6-14. For each, a sterile pipette tip was dipped in the colony, and then used to streak a region of the new plate. A second sterile pipette tip was used to streak out of this region into an unstreaked section. Streaked plates were placed in incubator @ 37C to grow.
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|2. '''Begin 2mL glycerol stock culture:'''  
|2. '''Begin 2mL glycerol stock culture:'''  
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|3. '''Transformation results'''
|3. '''Transformation results'''
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[[Image:DSCN2096.JPG|300px||center|thumb|Transformation reults]]
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|4. '''2mL cultures for plasmid miniprep'''
|4. '''2mL cultures for plasmid miniprep'''
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Latest revision as of 19:16, 1 July 2008

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Transformation reults
1. Streak LB + antibiotic plate:
a. From colonies whose plasmids we’ve sequenced, pick same colony and streak plates.Plates 6-14 were placed in the incubator @ 37C to regrow picked colonies for approximately 3 hours.
b. New LB plates with ampicillin were streaked with cells from the picked colonies for Biobricks 6-14. For each, a sterile pipette tip was dipped in the colony, and then used to streak a region of the new plate. A second sterile pipette tip was used to streak out of this region into an unstreaked section. Streaked plates were placed in incubator @ 37C to grow.
2. Begin 2mL glycerol stock culture:
a. The same colonies that were picked for the initial plasmid prep and the streaked plates directly above were again picked with a sterile pipette tip, and the tip was placed in 2mL LB broth + antibiotic (1uL/mL). All tubes were flamed immediately after opening and before closing and work was done near a flame to prevent contamination.
b. These were grown for approximately 18 hours @ 37C with shaking @ 220 rpm's.
3. Transformation results
4. 2mL cultures for plasmid miniprep