Minnesota/26 June 2008

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Revision as of 15:44, 1 July 2008

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1. Glycerol Stock:
a. Add 900uL of glycerol to empty tube.
b. Add 900uL of cells to the glycerol tubes.
c. Immediately place tubes in a beaker that has ethanol and dry ice for "snap freeze". Allow to freeze for 2 minutes.
d. Place tubes in freezer @ -79C for future use.