Newcastle University Wetlab/27 August 2008
From 2008.igem.org
Newcastle University
GOLD MEDAL WINNER 2008
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Wet lab work was carried out from 4 August to 19 September, Mondays to Fridays. Please click on a day to see the lab notebook.
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Wednesday 27th August
- ON cultures were analysed:
- pGFP-rrnB grown on ampicillin - cells had lysed (medium was clear with large bubbles at the surface).
- pJWV021 grown on ampicillin - frozen glycerol stock made (see protocol).
- pUC57-ncl08 grown on ampicillin - frozen glycerol stock made.
- A colony stab was taken of pGFP-rrnB using 10μl spectinomycin in 10mL LB as the culture medium.