Project
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- | <td colspan="2" bgcolor="#03438A" class="subHeader"><div align="center" class="STYLE16" style="margin-bottom: 0"> | + | <td height="124" colspan="2" bgcolor="#03438A" class="subHeader"><div align="center" class="STYLE16" style="margin-bottom: 0"> |
- | <p> | + | <p>PROJECT 1 </p> |
<p>A Foolproof Plasmid Self-Assembly system</p> | <p>A Foolproof Plasmid Self-Assembly system</p> | ||
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- | <td | + | <td width="954" height="39" bgcolor="#03438A"><span class="STYLE16" style="margin-bottom: 0"><strong><a href="https://2008.igem.org/B11111">Background</a></strong></span></td> |
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<p class="STYLE8">The recombinant DNA then could be selected in the liquid culture containing both ampicillin and kanamycin. Then, under inducible conditions, Cre will be expressed and the recombined sequence will be divided into two separate plasmids; one will retain the desired gene 1, while the other will preserve the killer gene ccdB, which is under the control of another inducible promoter. Because the two plasmids have shared origin site, plasmid incompatibility will occur thus the two kinds of plasmids will be separated into different cells. </p> | <p class="STYLE8">The recombinant DNA then could be selected in the liquid culture containing both ampicillin and kanamycin. Then, under inducible conditions, Cre will be expressed and the recombined sequence will be divided into two separate plasmids; one will retain the desired gene 1, while the other will preserve the killer gene ccdB, which is under the control of another inducible promoter. Because the two plasmids have shared origin site, plasmid incompatibility will occur thus the two kinds of plasmids will be separated into different cells. </p> | ||
<p class="STYLE8">When induced, CcdB could be expressed so that cells containing CcdB will be killed. </p> | <p class="STYLE8">When induced, CcdB could be expressed so that cells containing CcdB will be killed. </p> | ||
- | <p class="STYLE8">In order to realize the linkage of GENE 1 with GENE 2, we will introduce the new plasmid containing the desired GENE2 to the survival cells, in which the plasmids containing GENE 1 will behave as the new Receptor plasmid. Very similarly recombination between the <em>attB2 </em>and<em> attP2 </em>and the cleavage between the two <em>loxp </em> | + | <p class="STYLE8">In order to realize the linkage of GENE 1 with GENE 2, we will introduce the new plasmid containing the desired GENE2 to the survival cells, in which the plasmids containing GENE 1 will behave as the new Receptor plasmid. Very similarly recombination between the <em>attB2 </em>and<em> attP2 </em>and the cleavage between the two <em>loxp </em> sites will be performed, and plasmids containing the linked GENE1 and GENE2 will be selected when the promoter expresses CcdB is induced. </p> |
<p class="STYLE8">The reason for us to use two sets of<em> attB/attP</em> specific sites is to avoid the combination within one molecule. </p> | <p class="STYLE8">The reason for us to use two sets of<em> attB/attP</em> specific sites is to avoid the combination within one molecule. </p> | ||
<p class="STYLE15"> </p></td> | <p class="STYLE15"> </p></td> | ||
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- | <td height="59" colspan="2" bgcolor="#03438A" class="subHeader"><p align="center" class="STYLE3" style="margin-bottom: 0">The synthetic convertible ecosystem</p> | + | <td height="59" colspan="2" bgcolor="#03438A" class="subHeader"><p align="center" class="STYLE3" style="margin-bottom: 0">PROJECT 2 </p> |
+ | <p align="center" class="STYLE3">The synthetic convertible ecosystem</p></td> | ||
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- | <td height=" | + | <td height="141" colspan="2" bgcolor="#03438A" class="subHeader STYLE5"><p class="STYLE25"><span class="STYLE12"><a href="https://2008.igem.org/B22222">Background</a></span><br /> |
There is no mono-culture in nature! And in industry, coculture of species/strains are widely used to either improve productivity or lower the cost. The manufacturing of Vitamin C in China, which has contributed to 60 percent of its world production, could serve as an excellent example to validate the significance of coculture in industry. Thus to understand the interactions between coexistent ecosystems will not only contribute to human’s perception of nature but also to human practices in engineering.</p> | There is no mono-culture in nature! And in industry, coculture of species/strains are widely used to either improve productivity or lower the cost. The manufacturing of Vitamin C in China, which has contributed to 60 percent of its world production, could serve as an excellent example to validate the significance of coculture in industry. Thus to understand the interactions between coexistent ecosystems will not only contribute to human’s perception of nature but also to human practices in engineering.</p> | ||
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<td width="408" bgcolor="#03438A"><p class="STYLE19"><strong>Competition</strong></p> | <td width="408" bgcolor="#03438A"><p class="STYLE19"><strong>Competition</strong></p> | ||
- | <p class="STYLE15"> In the culture that both ampicillin and chloromycetin are available, it requires the expression of the both the resistant genes for both antibiotics for a strain’s survival. Without adding any signal molecular as the initially inducing factor into the culture the two kinds of E.coli can not communicate with each other so they will keep on the competent stage. In this stage each kind of cell must survive all by it self in some method as assimilating the nutrition in the culture. As a result the two different kinds of E.coli fight with each other for the space and nutrient ingredient. | + | <p class="STYLE15"> In the culture that both ampicillin and chloromycetin are available, it requires the expression of the both the resistant genes for both antibiotics for a strain’s survival. Without adding any signal molecular as the initially inducing factor into the culture the two kinds of E.coli can not communicate with each other so they will keep on the competent stage. In this stage each kind of cell must survive all by it self in some method as assimilating the nutrition in the culture. As a result the two different kinds of E.coli fight with each other for the space and nutrient ingredient. </p> |
<p class="STYLE15">By adding some AHL into the culture, the LuxPr promoter will get activated by the AHL and LuxR complex. And then the expression product of the rhlI gene BHL will diffuse into the cell-two which can sense BHL-RhIR complex by binding to the PrhI promoter and turning on the expression of luxI kanR and lacI genes. The LacI protein will bind to the PBad/araC promoter and therefore stop the digestion of the signal molecular by the expression of the aiiA gene. At the same time the expression of the luxI gene will send out AHL .By using a very similar mechanism the cell-one can sense the AHL molecular.</p></td> | <p class="STYLE15">By adding some AHL into the culture, the LuxPr promoter will get activated by the AHL and LuxR complex. And then the expression product of the rhlI gene BHL will diffuse into the cell-two which can sense BHL-RhIR complex by binding to the PrhI promoter and turning on the expression of luxI kanR and lacI genes. The LacI protein will bind to the PBad/araC promoter and therefore stop the digestion of the signal molecular by the expression of the aiiA gene. At the same time the expression of the luxI gene will send out AHL .By using a very similar mechanism the cell-one can sense the AHL molecular.</p></td> | ||
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Revision as of 07:49, 29 October 2008
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This idea was inspired by the theory of Prisoner’s Dilemma. As in prisoners’ dilemma, the bacteria in our design are faced with two solutions for coexistence, they could either choose to cooperate with one another by providing inducers to express their partners’ antibiotics-resistance genes or they could take a foe strategy in which no cooperation is needed for both strains’ survival. |
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