Rensselaer/23 August 2008

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Promoter Group

Aplification of J61002 mRFP plasmid

Pick 2 colonies to 2 15 ml tubes with 5 mls 2XYT and 75 ug/ml ampicillin.

Grow @ 37C ON w/ shaking.

Fe Promoter Ligation

Looked @ ligation plates.

Semiconfluent, but several colonies expressed mRFP.

Pick two red colonies to 5 mls 2XYT and 75 ug/ml ampicillin.

Grow ON @ 37C w/ shaking.

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