User contributions
From 2008.igem.org
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- 15:09, 17 July 2008 (diff | hist) Virginia/17 July 2008 (→Goals)
- 14:59, 17 July 2008 (diff | hist) Virginia/17 July 2008 (→Goals)
- 14:50, 17 July 2008 (diff | hist) Virginia/17 July 2008 (→Goals)
- 14:27, 17 July 2008 (diff | hist) N Virginia/17 July 2008 (New page: ==Overnight Results== *only plate with R0010 +A4 grew which is the same result as from the night before **Which leads us to think our assemblies have some problem *the assembly of BP2 and ...)
- 14:57, 15 July 2008 (diff | hist) N Virginia/15 July 2008 (New page: ==Goals== *GET GFP EXPRESSION! *miniprep DNA **Screening plasmid (psb1A10) **Assembly 3 (I715039 and E0240) **BP 2 **BP 3 *Assemble parts **Repressible Promoter(R0040) and GFP(E0240) **Sta...) (top)
- 14:46, 15 July 2008 (diff | hist) Virginia/14 July 2008 (top)
- 19:34, 14 July 2008 (diff | hist) N Virginia/14 July 2008 (New page: ==Goals== *turn in time sheets! *Examine flourescent proteins under flourescence camera **psb1A10 **assembly 3 of i715039 and E0240 *make overnight broths of these flourescent protein cell...)
- 19:23, 14 July 2008 (diff | hist) Virginia/11 July 2008 (top)
- 14:40, 8 July 2008 (diff | hist) N Virginia/8 July 2008 (New page: ==Goals== *Prepare overnight broth of successful single colony of Promoter + RBS transformation *Plate Screening plasmid (psb1a10) when it arrives from iGem *Try again with ligation of RFP...)
- 14:26, 8 July 2008 (diff | hist) N Virginia/7 July 2008 (New page: ==Goals== *Buy plane tickets *Reserve hotel room *Promoter + RBS ligation *Promoter + RBS transformation and plating ==Results== *have been having problems with purification of small part...) (top)
- 14:52, 1 July 2008 (diff | hist) N Virginia/1 July 2008 (New page: ==Goals== *digest parts *do not purify parts *heat deactivate digestion enzyme *ligate *transform Notebook) (top)
- 14:49, 1 July 2008 (diff | hist) N Virginia/27 June 2008 (New page: ==Goals== *small broth of plated parts *minipreps of parts that are going to be assembled *redigest all the assembly parts *run gels to verify purified cut outs *if possible run 3A assembl...) (top)
- 14:39, 30 June 2008 (diff | hist) N Virginia/28 June 2008 (New page: *Take out incubated over night brothes *spin down cultures to pellets to store in freezer Notebook) (top)
- 14:37, 30 June 2008 (diff | hist) N Virginia/30 June 2008 (New page: ==Goals== *Order midiprep kit *miniprep ligation of BP1 and terminator (Assembly 2) *Digest ligation *run regular gel of ligation and large purified parts that will be used in other ligati...)
- 15:35, 27 June 2008 (diff | hist) m Virginia/26 June 2008 (top)
- 15:29, 27 June 2008 (diff | hist) Virginia/26 June 2008
- 15:26, 27 June 2008 (diff | hist) m Virginia/26 June 2008 (→Notes)
- 15:25, 27 June 2008 (diff | hist) N Virginia/26 June 2008 (New page: ==Goals== *small broth of plated terminator *large broth of E0240(RFP) and I715039(GFP) *mini prepe PRE RFP and RET GFP for another ligation *redigest 6 parts for the three ligations *pu...)
- 15:13, 27 June 2008 (diff | hist) Virginia/25 June 2008 (top)
- 14:33, 25 June 2008 (diff | hist) Virginia/25 June 2008
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