User contributions
From 2008.igem.org
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- 19:15, 22 September 2008 (diff | hist) N Utah State/19 September 2008 (New page: Extracted various promoter regions from electrophoresis gel with Qiagen kit, namely C for-B rev, 5'Cpro for-ATG rev, 5'Cpro for-SD rev, 5'Cpro for-TC rev, -35Cpro for-ATG rev, and -35 pha ...) (top)
- 19:06, 22 September 2008 (diff | hist) N Utah State/22 September 2008 (New page: Isolated plasmid DNA from I20260 and E0240 constructs.) (top)
- 20:30, 19 September 2008 (diff | hist) File:Logo.jpg (uploaded a new version of "Image:Logo.jpg": Reverted to version as of 21:51, 1 July 2008)
- 23:50, 3 September 2008 (diff | hist) N Utah State/3 September 2008 (New page: Linear DNA was extracted from electrophoresis gels for the following constructs: 1. phbA with prefix and suffix 2. phbB with p/s 3. -35 pro with p/s 4. -35/TC with p/s 5. phbB w/out ...) (top)
- 22:40, 27 August 2008 (diff | hist) N Utah State/27 August 2008 (New page: Restriction digestion and electrophoresis was performed for the following: With EcoR1 and Xba1: E0240, E0840, E0420, E0422, E0430, E0240 With Spe1 and Pst1: I712074, R0010, R0040) (top)
- 21:32, 15 August 2008 (diff | hist) N Utah State/15 August 2008 (New page: PCR was run for 16s primers for the H16 cells and M. KMS cells, and Xba1 and Spe1 digestion was performed on portions of all purified DNA; electrophoresis was run for all of these.) (top)
- 21:34, 14 August 2008 (diff | hist) N Utah State/14 August 2008 (New page: DNA isolation was performed for the nine constructs that had not grown before. Plans were made for performing PCR with new primers tomorrow.) (top)
- 21:32, 14 August 2008 (diff | hist) N Utah State/13 August 2008 (New page: Cultures were prepared for the nine cultures that did not grow.) (top)
- 21:31, 14 August 2008 (diff | hist) N Utah State/12 August 2008 (New page: DNA isolation was performed for several constructs, but cultures did not grow for nine of the constructs.) (top)
- 21:30, 14 August 2008 (diff | hist) N Utah State/11 August 2008 (New page: Students learned how to use the DNA isolation kit. Cultures were prepared for all 22 transformed constructs for DNA isolation tomorrow.) (top)
- 20:48, 6 August 2008 (diff | hist) N Utah State/6 August 2008 (New page: Due to negative PCR results, it was decided to re-inoculate H16 Ralstonia Eutropha cultures since there may have been contamination in the culture used during the past two days of PCR. Si...) (top)
- 20:45, 6 August 2008 (diff | hist) N Utah State/5 August 2008 (New page: PCR was again attempted with the same oligos, but the annealing stage was performed on a gradient in the thermocycler. The gradient ranged from 40 to 60 C. Electrophoresis showed negativ...) (top)
- 20:43, 6 August 2008 (diff | hist) N Utah State/4 August 2008 (New page: PCR was done for the phb A,B, and C genes individually and as a whole. Amplification of the various regions of the promoter was also attempted. Electrophoresis showed negative results ex...) (top)
- 19:50, 23 July 2008 (diff | hist) N Utah State/22 July 2008 (New page: We discussed the process of making primers to PCR out genes of interest and how to submit BioBricks. We also looked at Biology Workbench and NCBI to learn the process of analyzing gene se...) (top)
- 05:49, 22 July 2008 (diff | hist) Utah State/18 July 2008 (top)
- 01:58, 19 July 2008 (diff | hist) N Utah State/18 July 2008 (New page: DNA separation preparation was done using the Mini-Scale Preparation for DNA Sequencing by DTAB-DNA Precipitation protocol. Restriction enzyme digestion was done using Xba1 and Pst1. Ele...)
- 21:06, 17 July 2008 (diff | hist) N Utah State/17 July 2008 (New page: Mini-scale preparation for DNA sequencing by CTAB-DNA precipitation was performed for all constructs except I20260 (the full GFP construct sent later by iGEM) up to the point of pellet rem...) (top)
- 21:24, 16 July 2008 (diff | hist) Utah State/16 July 2008 (top)
- 21:22, 16 July 2008 (diff | hist) N Utah State/16 July 2008 (New page: The I20260 construct was mistakenly placed on Amp plates. The transformation was redone today and placed on Kan plates. At least one colony per construct was noted (excepting I20260) and...)
- 16:55, 15 July 2008 (diff | hist) N Utah State/15 July 2008 (New page: None of the eight transformations from yesterday worked. An ampicillin plate from the batch we made yesterday was streaked with previously successfully transformed with T7 luciferase E. c...) (top)
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