Team:BCCS-Bristol/Calendar-Notebook/16 July 2008

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Swarm motility comparison - None of the 0.5% agar showed growth, Overall MG1655 was possibly slightly larger than MC1000 but wasn't a great result. (in Cambridge 06' MC1000 had much greater motility than MG1655)

Observed the dye plates, they had dried out somewhat overnight, however the dyes has mixed well. carried out experiment again with whatman paper soaked in water in 15cm petri dishes to make a humid environment. this time also used swimming medium (see table below) instead of the 0.1% agar used before as this may be more like what we use in the final experiment.

As results on motility comparison weren't great decided to try again

Swimming Medium

In 100ml of water weight (g)
Potassium Phosphate0.1742
NaCl0.392
Glucose0.18016
EDTA0.003722
Tween -20 aka polysorbate 200.2 MICRO litres