Team:Chiba/Calendar-Home/18 October 2008
From 2008.igem.org
17 October 2008 <|> 19 October 2008
Team:Demo-Rs
17 October~
- Sender Wash
- Centrifuged 2 tubes containing(BBa_T9002(JW1908))at 20°C,3600rpm for 6min and discarded supernatant.
- Added 10mL LB-Amp to each tube.
- Repeated wash twice.
- Creating bacterial plates
- LB-Amp pre-cultured Sender(BBa_S03623(JW1908)) tube 1 (10mL) was mixed with LB-Amp-agar(50°C)(10ml)to produce sender containing bacterialplate-1.
- Lifted with nitrocellulose
- Receiver(BBa_T9002(JW1908))colony was transfered to a nitrocellulose filter and placed on each of Sender(BBa_S03623(JW1908))containing bacterial plate (1~3) and Sender-absent negative control plate (t=0). Determined the time required for the colonies to fluoresce depending on the bacterial concentration (100 and 1000-fold dilution).
- Method to detect fluorescence
- Plates cultured at 37°C were exposed to UV (312nm) light once every 30 minutes to observe GFP fluorescence.
results
0h 0.5h 1.0h 1.5h 2.0h