Team:Chiba/Calendar-Home/28 August 2008

From 2008.igem.org

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[http://partsregistry.org/Part:BBa_I7106 BBa_I7106](2007)
[http://partsregistry.org/Part:BBa_I7106 BBa_I7106](2007)
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生えてきたコロニーを10ml培養して'''[[Team:Chiba/protocol/DNA Purification/sigma|Mini prep]]'''(60mlで溶出)
+
'''[[Team:Chiba/protocol/DNA Purification/sigma|Mini prep]]'''
-->'''[[Team:Chiba/protocol/digestion|Digestion]]'''
-->'''[[Team:Chiba/protocol/digestion|Digestion]]'''
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<br>混ぜ表
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<br>
<table width="150" border="4" cellpadding="0" cellspacing="0" bordercolor="#000000">
<table width="150" border="4" cellpadding="0" cellspacing="0" bordercolor="#000000">
<td width="257"></td>
<td width="257"></td>
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<tr>
<tr>
<td>TOTAL</td>
<td>TOTAL</td>
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<td>10μl </td>
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<td>10&mu;l </td>
</tr>
</tr>
</table>
</table>
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'''[[Team:Chiba/protocol/digestion|Digestion]]''' 37°C、30分
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'''[[Team:Chiba/protocol/digestion|Digestion]]''' 37&deg;C、30min
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<BR>プラスミドの濃度は33.6ng/μl
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<BR>-->Concentration 33.6ng/&mu;l
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'''[[Team:Chiba/protocol/PCR|PCR]]'''
'''[[Team:Chiba/protocol/PCR|PCR]]'''
<BR>RBS＋CI
<BR>RBS＋CI
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<BR>混ぜ表
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<BR>
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<table width="150" border="4" cellpadding="0" cellspacing="0" bordercolor="#000000">
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<tr>
<tr>
<td>TOTAL</td>
<td>TOTAL</td>
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<td>50μl </td>
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<td>50&mu;l </td>
</tr>
</tr>
</table>
</table>
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<table width="150" border="4" cellpadding="0" cellspacing="0" bordercolor="#000000">
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<td width="100">PCR産物</td>
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<td width="100">sampleDNA</td>
<td>1</td>
<td>1</td>
<tr>
<tr>
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<table width="150" border="4" cellpadding="0" cellspacing="0" bordercolor="#000000">
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<td width="100">PCR産物</td>
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<td width="100">sampleDNA</td>
<td>1</td>
<td>1</td>
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</tr>
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-->バンド出ず。
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-->none
===Team:Communication===
===Team:Communication===

Latest revision as of 03:14, 30 October 2008

>Home | Notebook

27 August 2008 <|> 29 August 2008

Contents

Laboratory work

Team:Input

Transformation

[http://partsregistry.org/Part:BBa_I7106 BBa_I7106](2007) Mini prep

-->Digestion

double
dH2O 2
10×BSA 1
10×NE 1
EcoRI 0.5
PstI 0.5
DNA 5
TOTAL 10μl

Digestion 37°C、30min
-->Concentration 33.6ng/μl


PCR
RBS＋CI

DNA 1
FW primer 2.5
VR primer 2.5
dNTPmix 5
thermo pol buffer 5
DNA pol(VENT) 1
dH2O 34
TOTAL 50μl


Gel Check


sampleDNA 1
Dye 1
dH2O 4
TOTAL 6μl

-->Concentration:33.6ng/μl


VectorPCR
[http://partsregistry.org/Part:BBa_I7106 BBa_I7106]

DNA 1
FW primer 5
VR primer 5
dNTPmix 10
thermo pol buffer 10
DNA pol(VENT) 1
dH2O 68
TOTAL 100μl


Gel check


sampleDNA 1
Dye 1
dH2O 4
TOTAL 6μl

-->none

Team:Communication

(27/8)--->Mini prep
  1. insert:[http://partsregistry.org/Part:BBa_C0170 BBa_C0170] + vector:[http://partsregistry.org/Part:BBa_J04500 BBa_J04500]
  2. insert:[http://partsregistry.org/Part:BBa_C0178 BBa_C0178] + vector:[http://partsregistry.org/Part:BBa_J04500 BBa_J04500]


--->Digestion Test
  1. insert:[http://partsregistry.org/Part:BBa_C0170 BBa_C0170] + vector:[http://partsregistry.org/Part:BBa_J04500 BBa_J04500]
  2. insert:[http://partsregistry.org/Part:BBa_C0178 BBa_C0178] + vector:[http://partsregistry.org/Part:BBa_J04500 BBa_J04500]
Sample No. 3456
insert:C0170 + vector:J04500 1-1-
insert:C0178 + vector:J04500 -1-1
EcoRI 0.10.10.10.1
SpeI --0.10.1
Buffer 3 1111
BSA --11
dH2O 7.87.86.86.8
TOTAL 10μl10μl10μl10μl


--->(29/8)Gel Check
Chiba-0828.JPG
Sample No. 1,23~6
Sample DNA 110
Loading Dye 12
H2O 4-
TOTAL 6μl12μl
From left;
  1. 　-> OK
  2. 　-> OK
  3. 　-> OK
  4. 　-> OK
  5. 　-> OK
  6. 　-> OK

Team:Output

Time Responce(liquid)

Retrieved from "http://2008.igem.org/Team:Chiba/Calendar-Home/28_August_2008"