Team:Chiba/Calendar-Home/28 August 2008

From 2008.igem.org

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'''[[Team:Chiba/protocol/gelcheck|ゲルチェック]]'''
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'''[[Team:Chiba/protocol/gelcheck|Gel Check]]'''
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-->濃度は33.6ng/μl
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-->Concentration:33.6ng/μl
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ベクターの'''[[Team:Chiba/protocol/PCR|PCR]]'''
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Vector'''[[Team:Chiba/protocol/PCR|PCR]]'''
<br>[http://partsregistry.org/Part:BBa_I7106 BBa_I7106]
<br>[http://partsregistry.org/Part:BBa_I7106 BBa_I7106]
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<br>混ぜ表
 
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'''[[Team:Chiba/protocol/gelcheck|ゲルチェック]]'''
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'''[[Team:Chiba/protocol/gelcheck|Gel check]]'''
    
    
                
                

Revision as of 20:17, 29 October 2008

>Home | Notebook

27 August 2008 <|> 29 August 2008

Contents

Laboratory work

Team:Input

Transformation

[http://partsregistry.org/Part:BBa_I7106 BBa_I7106](2007) 生えてきたコロニーを10ml培養してMini prep(60mlで溶出)

-->Digestion
混ぜ表

double
dH2O 2
10×BSA 1
10×NE 1
EcoRI 0.5
PstI 0.5
DNA 5
TOTAL 10μl

Digestion 37°C、30分
プラスミドの濃度は33.6ng/μl


PCR
RBS＋CI
混ぜ表

DNA 1
FW primer 2.5
VR primer 2.5
dNTPmix 5
thermo pol buffer 5
DNA pol(VENT) 1
dH2O 34
TOTAL 50μl


Gel Check


PCR産物 1
Dye 1
dH2O 4
TOTAL 6μl

-->Concentration:33.6ng/μl


VectorPCR
[http://partsregistry.org/Part:BBa_I7106 BBa_I7106]

DNA 1
FW primer 5
VR primer 5
dNTPmix 10
thermo pol buffer 10
DNA pol(VENT) 1
dH2O 68
TOTAL 100μl


Gel check


PCR産物 1
Dye 1
dH2O 4
TOTAL 6μl

-->バンド出ず。

Team:Communication

(27/8)--->Mini prep
  1. insert:[http://partsregistry.org/Part:BBa_C0170 BBa_C0170] + vector:[http://partsregistry.org/Part:BBa_J04500 BBa_J04500]
  2. insert:[http://partsregistry.org/Part:BBa_C0178 BBa_C0178] + vector:[http://partsregistry.org/Part:BBa_J04500 BBa_J04500]


--->Digestion Test
  1. insert:[http://partsregistry.org/Part:BBa_C0170 BBa_C0170] + vector:[http://partsregistry.org/Part:BBa_J04500 BBa_J04500]
  2. insert:[http://partsregistry.org/Part:BBa_C0178 BBa_C0178] + vector:[http://partsregistry.org/Part:BBa_J04500 BBa_J04500]
Sample No. 3456
insert:C0170 + vector:J04500 1-1-
insert:C0178 + vector:J04500 -1-1
EcoRⅠ 0.10.10.10.1
SpeⅠ --0.10.1
Buffer 3 1111
BSA --11
dH2O 7.87.86.86.8
TOTAL 10101010


--->(29/8)Gel Check
Chiba-0828.JPG
Sample No. 1,23~6
Sample DNA 110
Loading Dye 12
H2O 4-
TOTAL 612
From left;
  1. 　-> OK
  2. 　-> OK
  3. 　-> OK
  4. 　-> OK
  5. 　-> OK
  6. 　-> OK

Team:Output

Time Responce(liquid)

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