Team:Chiba/Calendar-Home/29 August 2008
From 2008.igem.org
28 August 2008 <|> 30 August 2008
Contents |
Laboratory work
Team:Input
no work
Team:Communication
- --->Digestion Test
- BBa_R0010(2007)
- BBa_C0062(2007)
- BBa_C0179(2007)
- BBa_C0179(2006)
- BBa_S03156(2007)
- BBa_S03156(2006)
- BBa_S03158(2007)
- BBa_S03158(2006)
- BBa_S03160(2007)
- BBa_S03160(2006)
Sample No. | S1~10 | W1 | W2~10 |
Sample DNA | 1 | 4 | 2 |
PstI | - | 0.075 | 0.1 |
EcoRI | 0.1 | 0.075 | 0.1 |
Buffer 3 | 1 | 0.75 | 1 |
BSA | - | 0.75 | 1 |
dH2O | 7.9 | 4.35 | 5.8 |
TOTAL | 10μl | 10μl | 10μl |
- --->Gel Check
|
|
- (27/8)--->Gel Check
|
- BBa_I9026(2007)
- BBa_I9030(2007)
- BBa_S03154(2007)
- BBa_R0010(2006)
Sample No. | 1~3 | 4 |
Sample DNA | 100ng/μL×20 | 200ng/μL×30 |
PstI | 2 | 2 |
XbaI | 2 | - |
SpeI | - | 2 |
Buffer 2 | - | 5 |
Buffer 3 | 5 | - |
BSA | 5 | 5 |
dH2O | 16 | 6 |
TOTAL | 50μl | 50μl |
- --->Gel Check
|
|
|
|
- --->Gel Extract
- --->Zymo Clean
- ->15μL
- --->SAP
Sample No. 4 Sample DNA 15 SAP 2 SAP Buffer 3 dH2O 10 TOTAL 30μL
- --->Zymo Clean
- --->Suspension! (We got information, by the team-output, to the effect that the density of the sample is too low.)
Team:Output