Team:Chiba/Calendar-Home/31 August 2008

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30 August 2008 <|> 1 September 2008

Contents

Laboratory work

Team:Input

no work

Team:Communication

Transformation
Competent Cells : XL10Gold
  • [http://partsregistry.org/Part:BBa_K084007 BBa_K084007](Plac+RBS+LasI)
  • [http://partsregistry.org/Part:BBa_K084008 BBa_K084008](Plac+RBS+RhlI)
---> We saved these plates with a refrigerator.


Digestion
  1. [http://partsregistry.org/Part:BBa_I9026 BBa_I9026](2007)
  2. [http://partsregistry.org/Part:BBa_I9030 BBa_I9030](2006)
  3. [http://partsregistry.org/Part:BBa_S03154 BBa_S03154](2007)
  4. [http://partsregistry.org/Part:BBa_R0010 BBa_R0010](2007)
Sample No. 1~34
Sample DNA(μL)1210
PstⅠ(μL) 0.20.2
XbaⅠ(μL)0.2-
SpeⅠ(μL)-0.4
Buffer 2(μL) -1.5
Buffer 3(μL) 2-
BSA(μL) 21.5
dH2O(μL) 3.61.4
TOTAL(μL) 2015


--->(1/9)Gel Check


(30/8)--->Mini prep


--->Digestion test
  • [http://partsregistry.org/Part:BBa_K084008 BBa_K084008] Sample No.2~5
  • [http://partsregistry.org/Part:BBa_T9002 BBa_T9002]①、②
Sample Single Digesiton 2~5Double Digestion 2~5Single Digestion T9002①Single Digesiton T9002②Double Digestion T9002①②
Sample DNA(μL)13313
XbaⅠ(μL)0.10.10.10.10.1
SpeⅠ(μL)-0.1--0.1
Buffer 2(μL) 0.90.80.90.90.8
BSA(μL) 11111
dH2O(μL) 75575
TOTAL(μL) 1010101010


--->Gel Check
Chiba-0831-p87.JPG
Sample No. [http://partsregistry.org/Part:BBa_K084008 BBa_K084008] Sample No.2~5, [http://partsregistry.org/Part:BBa_T9002 BBa_T9002]①② Single & Double Digestion
Sample DNA(μL) 110
Loading Dye(μL) 12
dH2O(μL) 4-
TOTAL(μL) 612
From Left
  • Single Digestion [http://partsregistry.org/Part:BBa_K084008 BBa_K084008] 2~5
  • Single Digestion [http://partsregistry.org/Part:BBa_T9002 BBa_T9002]①、②
  • Double Digestion [http://partsregistry.org/Part:BBa_K084008 BBa_K084008] 2~5
  • Double Digestion [http://partsregistry.org/Part:BBa_T9002 BBa_T9002]①、②
  • [http://partsregistry.org/Part:BBa_T9002 BBa_T9002]①、②



Team:Output

Digestion

  • [http://partsregistry.org/Part:BBa_R0010 BBa_R0010]①
  • [http://partsregistry.org/Part:BBa_J52008 BBa_J52008]②
Sample No.
DNA(μL) 1010
SpeⅠ(μL) 10
PstⅠ(μL) 11
XbaⅠ(μL) 01
BSA(x100)(μL) 0.50.5
Buffer2(μL) 50
Buffer3(μL) 05
dH2O(μL) 28.528.5
TOTAL(μL) 50μl50μl

-->left for 3 hours at 37 degrees.

-->①20μl refine

-->②20μl refine