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Team:Chiba/Calendar-Home/8 September 2008
From 2008.igem.org
(Difference between revisions)
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[[Team:Chiba/protocol/ligation/dephosphorylation|SAP]] | [[Team:Chiba/protocol/ligation/dephosphorylation|SAP]] | ||
| - | *[http://partsregistry.org/Part:BBa_R0040 BBa_R0040] | + | *[http://partsregistry.org/Part:BBa_R0040 BBa_R0040](1) |
| - | *[http://partsregistry.org/Part:BBa_R0010 BBa_R0010] | + | *[http://partsregistry.org/Part:BBa_R0010 BBa_R0010](5) |
<table width="250" border="4" cellpadding="0" cellspacing="0" bordercolor="#000000"> | <table width="250" border="4" cellpadding="0" cellspacing="0" bordercolor="#000000"> | ||
<tr> | <tr> | ||
<td width="257">Sample No.</td> | <td width="257">Sample No.</td> | ||
| - | <td> | + | <td>1</td><td>5</td> |
</tr> | </tr> | ||
<tr> | <tr> | ||
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-->left at 65 degrees for 30 minutes. | -->left at 65 degrees for 30 minutes. | ||
| - | -->[[Team:Chiba/protocol/DNA_Purification/zymocleam|DNA purification]]( | + | -->[[Team:Chiba/protocol/DNA_Purification/zymocleam|DNA purification]](1:21μl,5:6μl) |
| Line 76: | Line 76: | ||
</table> | </table> | ||
| - | *vector:[http://partsregistry.org/Part:BBa_R0040 BBa_R0040] | + | *vector:[http://partsregistry.org/Part:BBa_R0040 BBa_R0040](1) 75ng/μl |
| - | *insert:[http://partsregistry.org/Part:BBa_S03158 BBa_S03158] | + | *insert:[http://partsregistry.org/Part:BBa_S03158 BBa_S03158](2) 33.6ng/μl |
| - | *insert:[http://partsregistry.org/Part:BBa_S03156 BBa_S03156] | + | *insert:[http://partsregistry.org/Part:BBa_S03156 BBa_S03156](3) 33.6ng/μl |
| - | *insert:[http://partsregistry.org/Part:BBa_S03160 BBa_S03160] | + | *insert:[http://partsregistry.org/Part:BBa_S03160 BBa_S03160](4) 38.4ng/μl |
| - | *vector:[http://partsregistry.org/Part:BBa_R0010 BBa_R0010] | + | *vector:[http://partsregistry.org/Part:BBa_R0010 BBa_R0010](5) 100ng/μl |
| - | *insert:[http://partsregistry.org/Part:BBa_J52008 BBa_J52008] | + | *insert:[http://partsregistry.org/Part:BBa_J52008 BBa_J52008](6) 33.6ng/μl |
<table width="250" border="4" cellpadding="0" cellspacing="0" bordercolor="#000000"> | <table width="250" border="4" cellpadding="0" cellspacing="0" bordercolor="#000000"> | ||
<tr> | <tr> | ||
<td width="257">Sample No.</td> | <td width="257">Sample No.</td> | ||
| - | <td> | + | <td>1&2</td><td>1&3</td><td>1&4</td><td>1</td><td>5&6</td><td>5</td> |
</tr> | </tr> | ||
<tr> | <tr> | ||
Revision as of 22:10, 29 October 2008
7 September 2008 <|> 9 September 2008
Contents |
Laboratory work
Team:Input
no work
Team:Communication
-->(7/9)
- --->Zymo Clean
- eluted with 15μL(?) of NFW.
Team:Output
| Sample No. | 1 | 5 | |
| DNA(digested sample)(μL) | 7 | 6 | |
| SAP(μL) | 2 | 2 | |
| SAP Buffer(μL) | 3 | 3 | |
| TOTAL(μL) | 30 | 30 |
-->left at 37 degrees for 1 hour.
-->left at 65 degrees for 30 minutes.
-->DNA purification(1:21μl,5:6μl)
-->Gel check
| Sample No. | each |
| DNA(digested sample)(μL) | 1 |
| Loading Dye(μL) | 1 |
| dH2O(μL) | 4 |
| TOTAL(μL) | 6 |
- vector:BBa_R0040(1) 75ng/μl
- insert:BBa_S03158(2) 33.6ng/μl
- insert:BBa_S03156(3) 33.6ng/μl
- insert:BBa_S03160(4) 38.4ng/μl
- vector:BBa_R0010(5) 100ng/μl
- insert:BBa_J52008(6) 33.6ng/μl
| Sample No. | 1&2 | 1&3 | 1&4 | 1 | 5&6 | 5 |
| DNA(vector)(μL) | 3 | 3 | 3 | 3 | 5 | 2.1 |
| DNA(insert)(μL) | 3 | 3 | 3 | - | 3.1 | - |
| Ligase(μL) | 1 | 1 | 1 | 1 | 1 | 1 |
| Ligase Buffer(x5)(μL) | 2 | 2 | 2 | 2 | 2 | 2 |
| dH2O(μL) | 1 | 1 | 1 | 4 | 8.9 | 4 |
| TOTAL(μL) | 10 | 10 | 10 | 10 | 20 | 10 |
