Team:Chiba/Calendar-Home/8 September 2008

From 2008.igem.org

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'''[[Team:Chiba/protocol/ligation/gelex|Gel Extract]]'''
'''[[Team:Chiba/protocol/ligation/gelex|Gel Extract]]'''
 +
{|align="justify"
 +
|[[Image:Chiba-0908.JPG|300px]]
 +
|
 +
:<table width="315" border="2" cellpadding="0" cellspacing="0" bordercolor="#000000">
 +
<tr>
 +
<td width="257">Sample DNA</td>
 +
<td>60</td>
 +
</tr>
 +
<tr>
 +
<td>Loading Dye</td>
 +
<td>12</td>
 +
</tr>
 +
<tr>
 +
<td>TOTAL</td>
 +
<td>72μL</td>
 +
</tr>
 +
</table>
 +
:From left;
 +
::[http://partsregistry.org/Part:BBa_ BBa_](2007)
 +
::100 ng / 1 well
 +
|}
 +
 +
{|align="justify"
 +
|[[Image:Chiba-0908-2.JPG]]
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|
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:<table width="315" border="2" cellpadding="0" cellspacing="0" bordercolor="#000000">
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<tr>
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<td width="257">Sample Name</td>
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<td>[http://partsregistry.org/Part:BBa_J04500 BBa_J04500]</td><td>[http://partsregistry.org/Part:BBa_C0161 BBa_C0161]</td>
 +
</tr>
 +
<tr>
 +
<td width="257">Sample DNA</td>
 +
<td>30</td><td>60</td>
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</tr>
 +
<tr>
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<td>Loading Dye</td>
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<td>6</td><td>12</td>
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</tr>
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<tr>
 +
<td>TOTAL</td>
 +
<td>36μL</td><td>72μL</td>
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</tr>
 +
</table>
 +
:From left;
 +
::[http://partsregistry.org/Part:BBa_J04500 BBa_J04500]
 +
::[http://partsregistry.org/Part:BBa_C0161 BBa_C0161]
 +
|}
 +
 +
{|align="justify"
 +
|[[Image:Chiba-0908-3.JPG]]
 +
|
 +
:<table width="315" border="2" cellpadding="0" cellspacing="0" bordercolor="#000000">
 +
<tr>
 +
<td width="257">Sample DNA</td>
 +
<td>100</td>
 +
</tr>
 +
<tr>
 +
<td>Loading Dye</td>
 +
<td>20</td>
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</tr>
 +
<tr>
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<td>TOTAL</td>
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<td>6μL</td>
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</tr>
 +
</table>
 +
::[http://partsregistry.org/Part:BBa_C0261 BBa_C0261] -> none
 +
|}
:--->'''[[Team:Chiba/protocol/DNA Purification/zymocleam|Zymo Clean]]'''
:--->'''[[Team:Chiba/protocol/DNA Purification/zymocleam|Zymo Clean]]'''
:::eluted with 15μL(?) of NFW.
:::eluted with 15μL(?) of NFW.
 +
 +
 +
'''[https://2008.igem.org/Team:Chiba/protocol/Time_Delay_Test|Time Delay Test]'''
===Team:Output===
===Team:Output===
[[Team:Chiba/protocol/ligation/dephosphorylation|SAP]]
[[Team:Chiba/protocol/ligation/dephosphorylation|SAP]]
-
*[http://partsregistry.org/Part:BBa_R0040 BBa_R0040]①
+
*[http://partsregistry.org/Part:BBa_R0040 BBa_R0040](1)
-
*[http://partsregistry.org/Part:BBa_R0010 BBa_R0010]⑤
+
*[http://partsregistry.org/Part:BBa_R0010 BBa_R0010](5)
<table width="250" border="4" cellpadding="0" cellspacing="0" bordercolor="#000000">
<table width="250" border="4" cellpadding="0" cellspacing="0" bordercolor="#000000">
<tr>
<tr>
<td width="257">Sample No.</td>
<td width="257">Sample No.</td>
-
<td>①</td><td>⑤</td>
+
<td>1</td><td>5</td>
</tr>
</tr>
<tr>
<tr>
-
<td>DNA(digested sample)</td>
+
<td>DNA(digested sample)(μL)</td>
<td>7</td><td>6</td>
<td>7</td><td>6</td>
</tr>
</tr>
<tr>
<tr>
-
<td>SAP/td>
+
<td>SAP(μL)</td>
<td>2</td><td>2</td>
<td>2</td><td>2</td>
</tr>
</tr>
<tr>
<tr>
-
<td>SAP Buffer</td>
+
<td>SAP Buffer(μL)</td>
<td>3</td><td>3</td><td>
<td>3</td><td>3</td><td>
</tr>
</tr>
<tr>
<tr>
-
<td>TOTAL</td>
+
<td>TOTAL(μL)</td>
-
<td>30μl</td><td>30μl</td>
+
<td>30</td><td>30</td>
</tr>
</tr>
</table>
</table>
-
-->37℃ 1hour
+
-->left at 37 degrees for 1 hour.
-
-->65℃ 30min
+
-->left at 65 degrees for 30 minutes.
-
-->[[Team:Chiba/protocol/DNA_Purification/zymocleam|DNA purification]](①:21μl,⑤:6μl)
+
-->[[Team:Chiba/protocol/DNA_Purification/zymocleam|DNA purification]](1:21μl,5:6μl)
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</tr>
</tr>
<tr>
<tr>
-
<td>DNA(digested sample)</td>
+
<td>DNA(digested sample)(μL)</td>
<td>1</td>
<td>1</td>
</tr>
</tr>
<tr>
<tr>
-
<td>Loading Dye </td>
+
<td>Loading Dye(μL)</td>
<td>1</td>
<td>1</td>
</tr>
</tr>
<tr>
<tr>
-
<td>dH<sub>2</sub>O</td>
+
<td>dH<sub>2</sub>O(μL)</td>
<td>4</td>
<td>4</td>
</tr>
</tr>
<tr>
<tr>
-
<td>TOTAL</td>
+
<td>TOTAL(μL)</td>
-
<td>6μl</td>
+
<td>6</td>
</tr>
</tr>
</table>
</table>
-
*vector:[http://partsregistry.org/Part:BBa_R0040 BBa_R0040]① 75ng/μl
+
*vector:[http://partsregistry.org/Part:BBa_R0040 BBa_R0040](1) 75ng/μl
-
*insert:[http://partsregistry.org/Part:BBa_S03158 BBa_S03158]② 33.6ng/μl  
+
*insert:[http://partsregistry.org/Part:BBa_S03158 BBa_S03158](2) 33.6ng/μl  
-
*insert:[http://partsregistry.org/Part:BBa_S03156 BBa_S03156]③ 33.6ng/μl
+
*insert:[http://partsregistry.org/Part:BBa_S03156 BBa_S03156](3) 33.6ng/μl
-
*insert:[http://partsregistry.org/Part:BBa_S03160 Ba_S03160]④ 38.4ng/μl
+
*insert:[http://partsregistry.org/Part:BBa_S03160 BBa_S03160](4) 38.4ng/μl
-
*vector:[http://partsregistry.org/Part:BBa_R0010 BBa_R0010]⑤ 100ng/μl
+
*vector:[http://partsregistry.org/Part:BBa_R0010 BBa_R0010](5) 100ng/μl
-
*insert:[http://partsregistry.org/Part:BBa_J52008 BBa_J52008]⑥ 33.6ng/μl
+
*insert:[http://partsregistry.org/Part:BBa_J52008 BBa_J52008](6) 33.6ng/μl
<table width="250" border="4" cellpadding="0" cellspacing="0" bordercolor="#000000">
<table width="250" border="4" cellpadding="0" cellspacing="0" bordercolor="#000000">
<tr>
<tr>
<td width="257">Sample No.</td>
<td width="257">Sample No.</td>
-
<td>①&②</td><td>①&③</td><td>①&④</td><td>①</td><td>⑤&⑥</td><td>⑤</td>
+
<td>1&2</td><td>1&3</td><td>1&4</td><td>1</td><td>5&6</td><td>5</td>
</tr>
</tr>
<tr>
<tr>
-
<td>DNA(vector)</td>
+
<td>DNA(vector)(μL)</td>
<td>3</td><td>3</td><td>3</td><td>3</td><td>5</td><td>2.1</td>
<td>3</td><td>3</td><td>3</td><td>3</td><td>5</td><td>2.1</td>
</tr>
</tr>
<tr>
<tr>
-
<td>DNA(insert)</td>
+
<td>DNA(insert)(μL)</td>
<td>3</td><td>3</td><td>3</td><td>-</td><td>3.1</td><td>-</td>
<td>3</td><td>3</td><td>3</td><td>-</td><td>3.1</td><td>-</td>
</tr>
</tr>
<tr>
<tr>
-
<td>Ligase</td>
+
<td>Ligase(μL)</td>
<td>1</td><td>1</td><td>1</td><td>1</td><td>1</td><td>1</td>
<td>1</td><td>1</td><td>1</td><td>1</td><td>1</td><td>1</td>
</tr>
</tr>
<tr>
<tr>
-
<td>Ligase Buffer(x5)</td>
+
<td>Ligase Buffer(x5)(μL)</td>
<td>2</td><td>2</td><td>2</td><td>2</td><td>2</td><td>2</td>
<td>2</td><td>2</td><td>2</td><td>2</td><td>2</td><td>2</td>
</tr>
</tr>
<tr>
<tr>
-
<td>dH<sub>2</sub>O</td>
+
<td>dH<sub>2</sub>O(μL)</td>
<td>1</td><td>1</td><td>1</td><td>4</td><td>8.9</td><td>4</td>
<td>1</td><td>1</td><td>1</td><td>4</td><td>8.9</td><td>4</td>
</tr>
</tr>
<tr>
<tr>
-
<td>TOTAL</td>
+
<td>TOTAL(μL)</td>
-
<td>10μl</td><td>10μl</td><td>10μl</td><td>10μl</td><td>20μl</td><td>10μl</td>
+
<td>10</td><td>10</td><td>10</td><td>10</td><td>20</td><td>10</td>
</tr>
</tr>
</table>
</table>

Latest revision as of 23:43, 29 October 2008

>Home | Notebook

7 September 2008 <|> 9 September 2008

Contents

Laboratory work

Team:Input

no work

Team:Communication

-->(7/9)

Gel Extract

Chiba-0908.JPG
Sample DNA 60
Loading Dye 12
TOTAL 72μL
From left;
BBa_(2007)
100 ng / 1 well
Chiba-0908-2.JPG
Sample Name BBa_J04500BBa_C0161
Sample DNA 3060
Loading Dye 612
TOTAL 36μL72μL
From left;
BBa_J04500
BBa_C0161
Chiba-0908-3.JPG
Sample DNA 100
Loading Dye 20
TOTAL 6μL
BBa_C0261 -> none


--->Zymo Clean
eluted with 15μL(?) of NFW.


Delay Test

Team:Output

SAP

Sample No. 15
DNA(digested sample)(μL) 76
SAP(μL) 22
SAP Buffer(μL) 33
TOTAL(μL) 3030

-->left at 37 degrees for 1 hour.

-->left at 65 degrees for 30 minutes.

-->DNA purification(1:21μl,5:6μl)


-->Gel check

Sample No. each
DNA(digested sample)(μL) 1
Loading Dye(μL) 1
dH2O(μL) 4
TOTAL(μL) 6
Sample No. 1&21&31&415&65
DNA(vector)(μL) 333352.1
DNA(insert)(μL) 333-3.1-
Ligase(μL) 111111
Ligase Buffer(x5)(μL) 222222
dH2O(μL) 11148.94
TOTAL(μL) 101010102010
Retrieved from "http://2008.igem.org/Team:Chiba/Calendar-Home/8_September_2008"