Team:Chiba/Calendar-Home/8 September 2008

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:<table width="315" border="2" cellpadding="0" cellspacing="0" bordercolor="#000000">
:<table width="315" border="2" cellpadding="0" cellspacing="0" bordercolor="#000000">
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<tr>
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<td width="257">Sample Name</td>
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<td>[http://partsregistry.org/Part:BBa_J04500 BBa_J04500]</td><td>[http://partsregistry.org/Part:BBa_C0161 BBa_C0161]</td>
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</tr>
<tr>
<tr>
<td width="257">Sample DNA</td>
<td width="257">Sample DNA</td>
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|[[Image:Chiba-0908-3.JPG]]
|[[Image:Chiba-0908-3.JPG]]
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:<table width="315" border="2" cellpadding="0" cellspacing="0" bordercolor="#000000">
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<tr>
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<td width="257">Sample DNA</td>
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<td>100</td>
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</tr>
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<tr>
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<td>Loading Dye</td>
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<td>20</td>
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</tr>
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<tr>
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<td>TOTAL</td>
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<td>6μL</td>
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</tr>
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</table>
::[http://partsregistry.org/Part:BBa_C0261 BBa_C0261] -> none
::[http://partsregistry.org/Part:BBa_C0261 BBa_C0261] -> none
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:--->'''[[Team:Chiba/protocol/DNA Purification/zymocleam|Zymo Clean]]'''
:--->'''[[Team:Chiba/protocol/DNA Purification/zymocleam|Zymo Clean]]'''
:::eluted with 15μL(?) of NFW.
:::eluted with 15μL(?) of NFW.
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'''[https://2008.igem.org/Team:Chiba/protocol/Time_Delay_Test|Time Delay Test]'''
===Team:Output===
===Team:Output===

Latest revision as of 23:43, 29 October 2008

>Home | Notebook

7 September 2008 <|> 9 September 2008

Contents

Laboratory work

Team:Input

no work

Team:Communication

-->(7/9)

Gel Extract

Chiba-0908.JPG
Sample DNA 60
Loading Dye 12
TOTAL 72μL
From left;
BBa_(2007)
100 ng / 1 well
Chiba-0908-2.JPG
Sample Name BBa_J04500BBa_C0161
Sample DNA 3060
Loading Dye 612
TOTAL 36μL72μL
From left;
BBa_J04500
BBa_C0161
Chiba-0908-3.JPG
Sample DNA 100
Loading Dye 20
TOTAL 6μL
BBa_C0261 -> none


--->Zymo Clean
eluted with 15μL(?) of NFW.


Delay Test

Team:Output

SAP

Sample No. 15
DNA(digested sample)(μL) 76
SAP(μL) 22
SAP Buffer(μL) 33
TOTAL(μL) 3030

-->left at 37 degrees for 1 hour.

-->left at 65 degrees for 30 minutes.

-->DNA purification(1:21μl,5:6μl)


-->Gel check

Sample No. each
DNA(digested sample)(μL) 1
Loading Dye(μL) 1
dH2O(μL) 4
TOTAL(μL) 6
Sample No. 1&21&31&415&65
DNA(vector)(μL) 333352.1
DNA(insert)(μL) 333-3.1-
Ligase(μL) 111111
Ligase Buffer(x5)(μL) 222222
dH2O(μL) 11148.94
TOTAL(μL) 101010102010
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