Team:Chiba/Experiments:LuxR mutant
From 2008.igem.org
Home | The Team | The Project | Parts Submitted to the Registry | Reference | Notebook | Acknowledgements |
---|
LuxR mutant (Under Planning)
Design
Collins et.al. described the hyper-sensitive variants of luxR to AHL.(1) CollonsらとKockらによって、LuxRに変異を入れることでLuxRのAHLに対する感受性を変えることができると報告されている。(1),(2)
Fig. 3, Fig. 4 Hypothetical positions of residues in TraR corresponding to those found to modulate acyl-HSL specificity in LuxR. The crystal structure of the LuxR homologue TraR (PDB 1L3L) has been determined (Zhang et al., 2002).
ExperimentSender; LuxI plasmid transformed into E.coli strains (JW1908) Receiver; LuxR-gfp plasmid transformed into E.coli strains (JW1908) Culture/ Cndn.
Reference
|