Team:Chiba/Receiver

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__NOTOC__
__NOTOC__
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== Receiver strategy==
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== Signal Molecule Receiver==
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==Quorum_Sensing_Crosstalk==
==Quorum_Sensing_Crosstalk==
===Design===
===Design===
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[[Image:Table R chiba.gif|frame|left|Table.1 LuxR family gene]]
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センダーとレシーバーを元来とは異なる組み合わせにすることによって、thresholdに達する時間を遅くする。(杉山)
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===Experiment===
===Experiment===
==Plasmid_Copy_number==
==Plasmid_Copy_number==
===Design===
===Design===
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===Experiment===
===Experiment===
== LuxR_mutant ==
== LuxR_mutant ==
===Design===
===Design===
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[[Image:Location-of-the-mutant-in-LuxR Chiba.gif|frame|right|'''Fig.6''' Location of the LuxR mutant in LuxR]]
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[[Image:Mutant-LuxR Chiba.gif|frame|right|'''Table.1''' Seisitivity of LuxR mutants. Data modified from Collins et.al. Mol. Microbiol. 55, 712–723 (2005)]]
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Collins et.al. described the hyper-sensitive variants of luxR to AHL.(Collins, C. H., Arnold, F. H. & Leadbetter, J. R. Directed evolution of Vibrio fischeri LuxR for increased sensitivity to a broad spectrum of acyl-homoserine lactones. Mol. Microbiol. 55, 712–723 (2005))
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私たちはmutated Receiverを用いることで、AHL感受性の違う2種類(WTと変異体)のレシーバーを用意し、delay-timeのバリエーションを増やした。
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(小林)
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===Experiment===
===Experiment===

Latest revision as of 20:40, 27 October 2008

Chiba-U.gif

Home The Team The Project Parts Submitted to the Registry Reference Notebook Acknowledgements

Signal Molecule Receiver

Quorum_Sensing_Crosstalk

Design

Table.1 LuxR family gene

センダーとレシーバーを元来とは異なる組み合わせにすることによって、thresholdに達する時間を遅くする。(杉山)

Experiment

Plasmid_Copy_number

Design

Experiment

LuxR_mutant

Design

Fig.6 Location of the LuxR mutant in LuxR
Table.1 Seisitivity of LuxR mutants. Data modified from Collins et.al. Mol. Microbiol. 55, 712–723 (2005)


Collins et.al. described the hyper-sensitive variants of luxR to AHL.(Collins, C. H., Arnold, F. H. & Leadbetter, J. R. Directed evolution of Vibrio fischeri LuxR for increased sensitivity to a broad spectrum of acyl-homoserine lactones. Mol. Microbiol. 55, 712–723 (2005))

私たちはmutated Receiverを用いることで、AHL感受性の違う2種類(WTと変異体)のレシーバーを用意し、delay-timeのバリエーションを増やした。 (小林)

Experiment

Home The Team The Project Parts Submitted to the Registry Reference Notebook Acknowledgements