Team:Chiba/Sender experiments
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[[image:senders_crosstalk_chiba_01.gif|frame|left| | [[image:senders_crosstalk_chiba_01.gif|frame|left| | ||
'''Fig.''' senders crosstalk test.senders strain XL10Gold,Receiver strain JW1908.Reaction temparature was 30°C.All measurements are averages from three replicate cultures with error bars representing standard deviations.Labeling:LuxI,RhlI,LasI means fluorescence induced by AHLs synthesized by LuxI,RhlI,LasI respectively.]]<br clear="all"> | '''Fig.''' senders crosstalk test.senders strain XL10Gold,Receiver strain JW1908.Reaction temparature was 30°C.All measurements are averages from three replicate cultures with error bars representing standard deviations.Labeling:LuxI,RhlI,LasI means fluorescence induced by AHLs synthesized by LuxI,RhlI,LasI respectively.]]<br clear="all"> | ||
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+ | *軸ラベル、直します。 | ||
*RhlIとLuxIでは、GFP inductionにかかる時間はほとんど同じであった. | *RhlIとLuxIでは、GFP inductionにかかる時間はほとんど同じであった. |
Revision as of 07:08, 26 October 2008
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Contents |
Quorum-Sensing Cross-talk
Purpose
To Confirm that communication using non-endogenous molecules results in slower activation of receivers.
冨永
Senders
Receiver
Method
1.Crosstalk test
To characterize quorum sensing crosstalk, constitutive AHL senders were mixed with constitutive receivers and measure fluorescence intensity.
- Transformed Senders into E.coli strains(XL10GOLD) and Receiver into E.coli strain(JW1908).
- Inoculated them independently in liquid media. Incubated at 37°C 12h
- Mixed them.
- Incubated at 30°C.
- Measured intensity of green fluorescence at regular time intervals.
2.Demonstration
Result
Crosstalk test
- 軸ラベル、直します。
- RhlIとLuxIでは、GFP inductionにかかる時間はほとんど同じであった.
- LasIは、GFP inductionが他より約2時間遅れた.
(冨永)
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