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Team:Chiba/parts/BBa K084008
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===parts usage=== | ===parts usage=== | ||
[http://partsregistry.org/Part:BBa_K084008 BBa_K084008]([http://partsregistry.org/Part:BBa_J04500 plac+rbs]+[http://partsregistry.org/Part:BBa_C0170 RhlI(no LVA)]) | [http://partsregistry.org/Part:BBa_K084008 BBa_K084008]([http://partsregistry.org/Part:BBa_J04500 plac+rbs]+[http://partsregistry.org/Part:BBa_C0170 RhlI(no LVA)]) | ||
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[[Image:Tet-rhli chiba.gif]] | [[Image:Tet-rhli chiba.gif]] | ||
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日本語:IPTGにより、シュードモナス由来のC4HSL,C6HSL合成酵素,RhlIを発現するデバイス。 | 日本語:IPTGにより、シュードモナス由来のC4HSL,C6HSL合成酵素,RhlIを発現するデバイス。 | ||
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===Experient=== | ===Experient=== | ||
| + | ====Purpose==== | ||
To characterize quorum sensing crosstalk, constitutive AHL senders were mixed with constitutive receivers and measure fluorescence intensity. | To characterize quorum sensing crosstalk, constitutive AHL senders were mixed with constitutive receivers and measure fluorescence intensity. | ||
| - | + | ====Method==== | |
| - | #Transformed | + | #Transformed BBa_K084008 into E.coli strains(XL10GOLD or JW1908) and BBa_T9002 into E.coli strain(JW1908). |
#Inoculated them independently in liquid media. Incubated at 37°C 12h | #Inoculated them independently in liquid media. Incubated at 37°C 12h | ||
#Mixed them. | #Mixed them. | ||
| - | #Incubated at 30°C. | + | #Incubated at 30°C and 37°C. |
#Measured intensity of green fluorescence at regular time intervals. | #Measured intensity of green fluorescence at regular time intervals. | ||
| - | + | ||
| - | + | ====[[Team:Chiba/protocol/phenotype/timedelay|more details]]==== | |
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===Results=== | ===Results=== | ||
[[Image:BBa_K084007_XL10G_30_RS_1.gif|frame|left|Fig. ]] | [[Image:BBa_K084007_XL10G_30_RS_1.gif|frame|left|Fig. ]] | ||
Latest revision as of 11:52, 28 October 2008
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parts usage
[http://partsregistry.org/Part:BBa_K084008 BBa_K084008]([http://partsregistry.org/Part:BBa_J04500 plac+rbs]+[http://partsregistry.org/Part:BBa_C0170 RhlI(no LVA)])
日本語:IPTGにより、シュードモナス由来のC4HSL,C6HSL合成酵素,RhlIを発現するデバイス。
English:
Experient
Purpose
To characterize quorum sensing crosstalk, constitutive AHL senders were mixed with constitutive receivers and measure fluorescence intensity.
Method
- Transformed BBa_K084008 into E.coli strains(XL10GOLD or JW1908) and BBa_T9002 into E.coli strain(JW1908).
- Inoculated them independently in liquid media. Incubated at 37°C 12h
- Mixed them.
- Incubated at 30°C and 37°C.
- Measured intensity of green fluorescence at regular time intervals.
more details
Results
Fig.
