Team:Chiba/parts/BBa K084008

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parts usage

[http://partsregistry.org/Part:BBa_K084008 BBa_K084008]([http://partsregistry.org/Part:BBa_J04500 plac+rbs]+[http://partsregistry.org/Part:BBa_C0170 RhlI(no LVA)]) Tet-rhli chiba.gif

Tet-lasi chiba.gif

日本語:IPTGにより、シュードモナス由来のC4HSL,C6HSL合成酵素,RhlIを発現するデバイス。

English:

Experient

To characterize quorum sensing crosstalk, constitutive AHL senders were mixed with constitutive receivers and measure fluorescence intensity.

  1. Transformed BBa_K084008 into E.coli strains(XL10GOLD or JW1908) and BBa_T9002 into E.coli strain(JW1908).
  2. Inoculated them independently in liquid media. Incubated at 37°C 12h
  3. Mixed them.
  4. Incubated at 30°C.
  5. Measured intensity of green fluorescence at regular time intervals.
  1. E.Coli strain JW1908 or XL10Gold.
  2. Reaction Temparature 37 or 30.
  3. Cell number.

Results

Fig.
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