Team:Davidson-Missouri Western/Improvement of Pre-existing Registry Parts
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(New page: '''Improved function by a modified Lac promoter''' - Mutations were introduced into the wild type Lac promoter that exists in the Registry. One mutant promoter, designated pLacIQ1, had im...) |
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+ | {| style="color:#1b2c8a;background-color:#0c6;" cellpadding="3" cellspacing="1" border="1" bordercolor="#fff" width="62%" align="center" | ||
+ | !align="center"|[[Team:Davidson-Missouri_Western|Home]] | ||
+ | !align="center"|[[Team:Davidson-Missouri_Western/Team|The Team]] | ||
+ | !align="center"|[[Team:Davidson-Missouri_Western/Project|''E. nigma'' Project]] | ||
+ | !align="center"|[[Team:Davidson-Missouri_Western/New_Parts_Contributed_to_the_Registry|Parts Submitted to the Registry]] | ||
+ | !align="center"|[[Team:Davidson-Missouri_Western/Notebook|Notebook]] | ||
+ | |} | ||
'''Improved function by a modified Lac promoter''' - Mutations were introduced into the wild type Lac promoter that exists in the Registry. One mutant promoter, designated pLacIQ1, had improved ability to promote, as demonstrated by the following experiment. | '''Improved function by a modified Lac promoter''' - Mutations were introduced into the wild type Lac promoter that exists in the Registry. One mutant promoter, designated pLacIQ1, had improved ability to promote, as demonstrated by the following experiment. | ||
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- | Constructs were assembled with | + | Constructs were assembled with either the wild type Lac promoter or mutant promoters driving GFP expression via part number E0240. Fluorescence measurements taken at 16 or 24 hours were corrected for optical density. |
16 hours | 16 hours | ||
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'''Mutations were also introduced into the LacI repressor gene in order to improve its ability to bind to the operator site in the Lac promoter. | '''Mutations were also introduced into the LacI repressor gene in order to improve its ability to bind to the operator site in the Lac promoter. | ||
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|- | |- | ||
- | |BBa_K091121||LacI wild-type gene || || 1083 | + | |BBa_K091121||LacI wild-type gene ||pSB1A2 || 1083 |
|- | |- | ||
- | |BBa_K091122||LacI_I12 || || 1083 | + | |BBa_K091122||LacI_I12 || pSB1A2 || 1083 |
|- | |- | ||
- | |BBa_K091127||LacI_I12_X86 || || 1083 | + | |BBa_K091127||LacI_I12_X86 || pSB1A2 || 1083 |
|- | |- | ||
+ | |} | ||
+ | <br> | ||
+ | {| style="color:#1b2c8a;background-color:#0c6;" cellpadding="3" cellspacing="1" border="1" bordercolor="#fff" width="62%" align="center" | ||
+ | !align="center"|[[Team:Davidson-Missouri_Western|Home]] | ||
+ | !align="center"|[[Team:Davidson-Missouri_Western/Team|The Team]] | ||
+ | !align="center"|[[Team:Davidson-Missouri_Western/Project|''E. nigma'' Project]] | ||
+ | !align="center"|[[Team:Davidson-Missouri_Western/New_Parts_Contributed_to_the_Registry|Parts Submitted to the Registry]] | ||
+ | !align="center"|[[Team:Davidson-Missouri_Western/Notebook|Notebook]] | ||
|} | |} |
Latest revision as of 03:40, 30 October 2008
Home | The Team | E. nigma Project | Parts Submitted to the Registry | Notebook |
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Improved function by a modified Lac promoter - Mutations were introduced into the wild type Lac promoter that exists in the Registry. One mutant promoter, designated pLacIQ1, had improved ability to promote, as demonstrated by the following experiment.
Constructs were assembled with either the wild type Lac promoter or mutant promoters driving GFP expression via part number E0240. Fluorescence measurements taken at 16 or 24 hours were corrected for optical density.
16 hours
Sample | Fluorescence | Optical Density | Fluor/OD | |
---|---|---|---|---|
LB | 2 | 0.043 | 46.5 | |
E0240 | 0.67 | 1.262 | 0.53 | |
S04020 | 4.67 | 1.225 | 3.81 | |
S04021 | 21 | 1.201 | 17.49 | |
K091131 | 68 | 1.188 | 57.24 |
24 hours
Sample | Fluorescence | Optical Density | Fluor/OD | |
---|---|---|---|---|
LB | 1 | 0.047 | 21.3 | |
E0240 | 3.0 | 1.201 | 2.50 | |
S04020 | 4.0 | 1.239 | 3.23 | |
S04021 | 14.5 | 1.216 | 11.92 | |
K091131 | 71.3 | 1.207 | 59.07 |
Mutations were also introduced into the LacI repressor gene in order to improve its ability to bind to the operator site in the Lac promoter.
Part | Description | plasmid | size |
---|---|---|---|
BBa_K091121 | LacI wild-type gene | pSB1A2 | 1083 |
BBa_K091122 | LacI_I12 | pSB1A2 | 1083 |
BBa_K091127 | LacI_I12_X86 | pSB1A2 | 1083 |
Home | The Team | E. nigma Project | Parts Submitted to the Registry | Notebook |
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