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Team:EPF-Lausanne/Parts
From 2008.igem.org
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===Parts submitted to the registry=== | ===Parts submitted to the registry=== | ||
| - | [http://partsregistry.org/Part:BBa_K092000 | + | [http://partsregistry.org/Part:BBa_K092000 BBa_K092000]: <br> |
| - | BBa_K092000 : <br> | + | |
Composition : B0034 + C0040 + B0010 + B0012 = RBS + TetR with LVA + stop + stop<br> | Composition : B0034 + C0040 + B0010 + B0012 = RBS + TetR with LVA + stop + stop<br> | ||
TetR coding sequence with RBS and terminator : | TetR coding sequence with RBS and terminator : | ||
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| - | [http://partsregistry.org/Part:BBa_K092100 | + | [http://partsregistry.org/Part:BBa_K092100 BBa_K092100] :<br> |
| - | BBa_K092100 : | + | |
Composition : I14033 + B0034 + C0171 + B0010 + B0012 = Constutive promoter + RBS + RhlR + stop + stop<br> | Composition : I14033 + B0034 + C0171 + B0010 + B0012 = Constutive promoter + RBS + RhlR + stop + stop<br> | ||
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| - | [http://partsregistry.org/Part:BBa_K092200 | + | [http://partsregistry.org/Part:BBa_K092200 BBa_K092200] : |
| - | BBa_K092200 : | + | |
Composition : B0034 + C0070 + B0010 + B0012 = RBS + RhlI + stop + stop<br> | Composition : B0034 + C0070 + B0010 + B0012 = RBS + RhlI + stop + stop<br> | ||
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| - | [http://partsregistry.org/Part:BBa_K092300 | + | [http://partsregistry.org/Part:BBa_K092300 BBa_K092300] : |
| - | BBa_K092300 : | + | |
Composition : R0040 + B0034 + E1010 = TetR repressible promoter + RBS + RFP<br> | Composition : R0040 + B0034 + E1010 = TetR repressible promoter + RBS + RFP<br> | ||
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| - | [http://partsregistry.org/Part:BBa_K092400 | + | [http://partsregistry.org/Part:BBa_K092400 BBa_K092400] : |
| - | BBa_K092400 : | + | |
New part created by 2-steps PCR. RBS (B0034) + LuxI + Terminator (B0010+B0012) : | New part created by 2-steps PCR. RBS (B0034) + LuxI + Terminator (B0010+B0012) : | ||
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| - | [http://partsregistry.org/Part:BBa_K092600 | + | [http://partsregistry.org/Part:BBa_K092600 BBa_K092600] : |
| - | BBa_K092600 : | + | |
Composition : B0034 + C0040 + B0010 + B0012 + R0040 + B0034 + E1010 = RBS + TetR with LVA + stop + stop + TetR repressible promoter + RBS + RFP<br> | Composition : B0034 + C0040 + B0010 + B0012 + R0040 + B0034 + E1010 = RBS + TetR with LVA + stop + stop + TetR repressible promoter + RBS + RFP<br> | ||
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| - | [http://partsregistry.org/Part:BBa_K092700 | + | [http://partsregistry.org/Part:BBa_K092700 BBa_K092700] : |
| - | BBa_K092700 : | + | |
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| - | [http://partsregistry.org/Part:BBa_K092800 | + | [http://partsregistry.org/Part:BBa_K092800 BBa_K092800] : |
| - | BBa_K092800: | + | |
Coding sequence for LacI modified with a different rate of translation than LacI, RBS. | Coding sequence for LacI modified with a different rate of translation than LacI, RBS. | ||
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| - | [http://partsregistry.org/Part:BBa_K092900 | + | [http://partsregistry.org/Part:BBa_K092900 BBa_K092900] : |
| - | BBa_K092900: | + | |
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| - | [http://partsregistry.org/Part:BBa_K092901 | + | [http://partsregistry.org/Part:BBa_K092901 BBa_K092901]: Project Part |
| - | BBa_K092901: Project Part | + | |
Revision as of 18:51, 28 October 2008
Cloning Scheme
First plasmid : the basic network components
Second plasmid : the testing component
Parts submitted to the registry
BBa_K092000:
Composition : B0034 + C0040 + B0010 + B0012 = RBS + TetR with LVA + stop + stop
TetR coding sequence with RBS and terminator :
Coding sequence of the Tetracycline repressor with the ribosome binding site and a double terminator. The TetR comes from transposon Tn10 and has a LVA tail (SsrA) [cf. C0040 part].
Composition : I14033 + B0034 + C0171 + B0010 + B0012 = Constutive promoter + RBS + RhlR + stop + stop
Constitutive RhlR Receiver
Composition : B0034 + C0070 + B0010 + B0012 = RBS + RhlI + stop + stop
RhlI with RBS and terminator
Composition : R0040 + B0034 + E1010 = TetR repressible promoter + RBS + RFP
RFP controlled by a TetR promoter :
Coding sequence of the RFP with a ribosome binding site and pTetR as promoter. Thus, the transcription of RFP is inhibited when TetR is present.
BBa_K092400 :
New part created by 2-steps PCR. RBS (B0034) + LuxI + Terminator (B0010+B0012) :
Coding sequence of the Lux Inducer with a ribosome binding site and terminator. This biobrick is an alternative to the F1610 which didn't work
Composition : B0034 + C0040 + B0010 + B0012 + R0040 + B0034 + E1010 = RBS + TetR with LVA + stop + stop + TetR repressible promoter + RBS + RFP
TetR cds with RBS and Terminator + pTetR, RFP with RBS
The TetR sequence (with a ribosome binding site and terminator) is bound to the part containing the promoter of TetR, a ribosome binding site and the red fluorescent protein (RFP). Thus, the production of RFP directly depends on the production of TetR. The transcription of RFP is inhibited when TetR is present. The production of TetR depends on the promoter you put in front of it.
Composition : B0034 + C0040 + B0010 + B0012 + R0040 + B0034 + E1010 + K092400 = RBS + TetR with LVA + stop + stop + TetR repressible promoter + RBS + RFP + RBS +LuxI + stop+stop
TetR cds with RBS and Terminator + pTetR, RFP with RBS + LuxI with RBS and terminator
The TetR sequence (with a ribosome binding site and terminator)BBa_K092000 is bound to the part containing the promoter of TetR and the red fluorescent protein (RFP) with a ribosome binding site. A luxI part (with RBS and terminator) is added at the end of the cds of the RFP. As no terminator was placed after the RFP, they both depends on the TetR promoter. The transcription of RFP and LuxI is inhibited when TetR is present. The production of TetR depends on the promoter you put in front of it.
Coding sequence for LacI modified with a different rate of translation than LacI, RBS.
Source of LacIm : Basu et al."A synthetic multicelluar system for programmed pattern formation", Nature. 2005 Apr 28;434(7037):1130-4
Composition : I14033 + I1466 + R0071 + K092800 = Constutive promoter + RBS + RhlR + Terminator + RBS + LacIm
RhlR controlled by a constitutive promoter, and LacI modified with a Rhl R promoter.
BBa_K092901: Project Part
Composition : I14033 + I1466 + R0071 + K092800 + B0015 + R0010 + B0034 + C0040 + B0010 + B0012 + R0040 + B0034 + E1010 + K092400
Sequence containing : - RhlR controlled by a constitutive promoter - LacI modified with a RhlR promoter - TetR controlled by a Lac Promoter - RFP controlled by a TetR promoter - LuxI is added at the end of the cds of the RFP. As no terminator was placed after the RFP, they both depends on the TetR promoter. The transcription of RFP and LuxI is inhibited when TetR is present. The production of TetR depends on the promoter you put in front of it.
