Team:Freiburg/Parts
From 2008.igem.org
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*'''Tm-Fluo-linker-cYFP'''<br> | *'''Tm-Fluo-linker-cYFP'''<br> | ||
'''Part Name:'''<br> | '''Part Name:'''<br> | ||
- | [http://partsregistry.org/wiki/index.php?title=Part:BBa_K157014 BBa_K157014] | + | [http://partsregistry.org/wiki/index.php?title=Part:BBa_K157014 BBa_K157014]<br> |
'''Description:'''<br> | '''Description:'''<br> | ||
C-terminal part of the Split fluorophore YFP fused to the trans-membrane region of the Erbb1/EGF-receptor with the fluolinker in between. | C-terminal part of the Split fluorophore YFP fused to the trans-membrane region of the Erbb1/EGF-receptor with the fluolinker in between. | ||
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*'''Tm-nYFP'''<br> | *'''Tm-nYFP'''<br> | ||
'''Part Name:'''<br> | '''Part Name:'''<br> | ||
- | [http://partsregistry.org/wiki/index.php?title=Part:BBa_K157015 BBa_K157015] | + | [http://partsregistry.org/wiki/index.php?title=Part:BBa_K157015 BBa_K157015]<br> |
'''Description:'''<br> | '''Description:'''<br> | ||
N-terminal part of the Split-flourophore YFP, fused to the trans-membrane region of the Erbb1/EGF-receptor. | N-terminal part of the Split-flourophore YFP, fused to the trans-membrane region of the Erbb1/EGF-receptor. | ||
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*'''Tm-Fluolinker-cCFP'''<br> | *'''Tm-Fluolinker-cCFP'''<br> | ||
'''Part Name:'''<br> | '''Part Name:'''<br> | ||
- | [http://partsregistry.org/wiki/index.php?title=Part:BBa_K157016 BBa_K157016] | + | [http://partsregistry.org/wiki/index.php?title=Part:BBa_K157016 BBa_K157016]<br> |
'''Description:'''<br> | '''Description:'''<br> | ||
C-terminal part of Split-CFP, fused to the trans-membrane region of the Erbb1/EGF-receptor with the fluolinker in between. | C-terminal part of Split-CFP, fused to the trans-membrane region of the Erbb1/EGF-receptor with the fluolinker in between. | ||
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*'''Tm-nCFP'''<br> | *'''Tm-nCFP'''<br> | ||
'''Part Name:'''<br> | '''Part Name:'''<br> | ||
- | [http://partsregistry.org/wiki/index.php?title=Part:BBa_K157017 BBa_K157017] | + | [http://partsregistry.org/wiki/index.php?title=Part:BBa_K157017 BBa_K157017]<br> |
'''Description:'''<br> | '''Description:'''<br> | ||
N-terminal part of Split-CFP, fused to the trans-membrane region of the Erbb1/EGF-receptor. | N-terminal part of Split-CFP, fused to the trans-membrane region of the Erbb1/EGF-receptor. | ||
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*'''Tm-betaLactamaseBla1''' | *'''Tm-betaLactamaseBla1''' | ||
'''Part Name:'''<br> | '''Part Name:'''<br> | ||
- | [http://partsregistry.org/wiki/index.php?title=Part:BBa_K157018 BBa_K157018] | + | [http://partsregistry.org/wiki/index.php?title=Part:BBa_K157018 BBa_K157018]<br> |
'''Description:'''<br> | '''Description:'''<br> | ||
First fragment of beta-Lactamase fused to the trans-membrane region of the Erbb1/EGF-receptor. | First fragment of beta-Lactamase fused to the trans-membrane region of the Erbb1/EGF-receptor. | ||
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*'''Tm-betaLactamase(Bla2)''' | *'''Tm-betaLactamase(Bla2)''' | ||
'''Part Name:'''<br> | '''Part Name:'''<br> | ||
- | [http://partsregistry.org/wiki/index.php?title=Part:BBa_K157019 BBa_K157019] | + | [http://partsregistry.org/wiki/index.php?title=Part:BBa_K157019 BBa_K157019]<br> |
'''Description:'''<br> | '''Description:'''<br> | ||
Second fragment of beta-Lactamase fused to the trans-membrane region of the Erbb1/EGF-receptor. | Second fragment of beta-Lactamase fused to the trans-membrane region of the Erbb1/EGF-receptor. | ||
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*'''Tm-Split-Luciferase1/2''' | *'''Tm-Split-Luciferase1/2''' | ||
'''Part Name:'''<br> | '''Part Name:'''<br> | ||
- | [http://partsregistry.org/wiki/index.php?title=Part:BBa_K157020 BBa_K157020] | + | [http://partsregistry.org/wiki/index.php?title=Part:BBa_K157020 BBa_K157020]<br> |
'''Description:'''<br> | '''Description:'''<br> | ||
Part 1 of the Split- Luciferase(BB057) fused to the trans-membrane region of the Erbb1/EGF-receptor. | Part 1 of the Split- Luciferase(BB057) fused to the trans-membrane region of the Erbb1/EGF-receptor. | ||
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*'''Tm-Splitluciferase2/2''' | *'''Tm-Splitluciferase2/2''' | ||
'''Part Name:'''<br> | '''Part Name:'''<br> | ||
+ | [http://partsregistry.org/wiki/index.php?title=Part:BBa_K157021 BBa_K157021]<br> | ||
'''Description:'''<br> | '''Description:'''<br> | ||
Second part of Split-Luciferase(BB058) fused to the trans-membrane region of the Erbb1/EGF-receptor. | Second part of Split-Luciferase(BB058) fused to the trans-membrane region of the Erbb1/EGF-receptor. | ||
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*'''SP-AntiNIP-GGGSlinker''' | *'''SP-AntiNIP-GGGSlinker''' | ||
'''Part Name:'''<br> | '''Part Name:'''<br> | ||
- | [http://partsregistry.org/wiki/index.php?title=Part:BBa_K157022 BBa_K157022] | + | [http://partsregistry.org/wiki/index.php?title=Part:BBa_K157022 BBa_K157022]<br> |
'''Description:'''<br> | '''Description:'''<br> | ||
The scFv-anti-NIP (with a flexible GGGS-linker at the C-terminus) fused to the signal peptide of Erbb1/EGF-receptor. | The scFv-anti-NIP (with a flexible GGGS-linker at the C-terminus) fused to the signal peptide of Erbb1/EGF-receptor. | ||
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*'''SP-Lipocalin-GGGSlinker''' | *'''SP-Lipocalin-GGGSlinker''' | ||
'''Part Name:'''<br> | '''Part Name:'''<br> | ||
- | [http://partsregistry.org/wiki/index.php?title=Part:BBa_K157023 BBa_K157023] | + | [http://partsregistry.org/wiki/index.php?title=Part:BBa_K157023 BBa_K157023]<br> |
'''Description:'''<br> | '''Description:'''<br> | ||
Lipocalin (with a flexible GGGS-linker at the C-terminus) fusioned to the signal peptide of Erbb1/EGF-receptor. | Lipocalin (with a flexible GGGS-linker at the C-terminus) fusioned to the signal peptide of Erbb1/EGF-receptor. | ||
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*SP-AntiNIP-GGGSlinker-Tm-betaLactamase(Bla1) | *SP-AntiNIP-GGGSlinker-Tm-betaLactamase(Bla1) | ||
'''Part Name:'''<br> | '''Part Name:'''<br> | ||
- | [http://partsregistry.org/wiki/index.php?title=Part:BBa_K157024 BBa_K157024] | + | [http://partsregistry.org/wiki/index.php?title=Part:BBa_K157024 BBa_K157024]<br> |
'''Description:'''<br> | '''Description:'''<br> | ||
A composite part consisting of the signal-peptide of Erbb1/EGF-receptor, scFV-antiNIP, GGGSflexlinker, the trans-membrane region of the Erbb1/EGF-receptor and the N-terminal part of split-enzyme beta-Lactamase. | A composite part consisting of the signal-peptide of Erbb1/EGF-receptor, scFV-antiNIP, GGGSflexlinker, the trans-membrane region of the Erbb1/EGF-receptor and the N-terminal part of split-enzyme beta-Lactamase. | ||
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*'''SP-AntiNIP-GGGSlinker-Tm-betaLactamase(Bla2)''' | *'''SP-AntiNIP-GGGSlinker-Tm-betaLactamase(Bla2)''' | ||
'''Part Name:'''<br> | '''Part Name:'''<br> | ||
- | [http://partsregistry.org/wiki/index.php?title=Part:BBa_K157025 BBa_K157025] | + | [http://partsregistry.org/wiki/index.php?title=Part:BBa_K157025 BBa_K157025]<br> |
'''Description:'''<br> | '''Description:'''<br> | ||
A composite part consisting of the signal peptide of Erbb1/EGF-receptor, scFV-antiNIP, GGGSflexlinker, the trans-membrane region of the Erbb1/EGF-receptor and the C-terminal part of the split-enzyme beta-Lactamase. | A composite part consisting of the signal peptide of Erbb1/EGF-receptor, scFV-antiNIP, GGGSflexlinker, the trans-membrane region of the Erbb1/EGF-receptor and the C-terminal part of the split-enzyme beta-Lactamase. | ||
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*'''SP-AntiNIP-GGGSlinker-Tm-Fluolinker-cYFP''' | *'''SP-AntiNIP-GGGSlinker-Tm-Fluolinker-cYFP''' | ||
'''Part Name:'''<br> | '''Part Name:'''<br> | ||
- | [http://partsregistry.org/wiki/index.php?title=Part:BBa_K157026 BBa_K157026] | + | [http://partsregistry.org/wiki/index.php?title=Part:BBa_K157026 BBa_K157026]<br> |
'''Description:'''<br> | '''Description:'''<br> | ||
A composite part consisting of the signal peptide of Erbb1/EGF-receptor, scFV-antiNIP, GGGSflexlinker, the trans-membrane region of the Erbb1/EGF-receptor and the C-terminal part of the split fluorophor YFP with a fluolinker joined to its N-Terminus. | A composite part consisting of the signal peptide of Erbb1/EGF-receptor, scFV-antiNIP, GGGSflexlinker, the trans-membrane region of the Erbb1/EGF-receptor and the C-terminal part of the split fluorophor YFP with a fluolinker joined to its N-Terminus. | ||
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'''gene sequence:'''<br><br> | '''gene sequence:'''<br><br> | ||
- | * | + | *'''SP-AntiNIP-GGGSlinker-Tm-nYFP''' |
'''Part Name:'''<br> | '''Part Name:'''<br> | ||
+ | [http://partsregistry.org/wiki/index.php?title=Part:BBa_K157027 BBa_K157027] | ||
'''Description:'''<br> | '''Description:'''<br> | ||
+ | Part consists of the signal peptide of Erbb1/EGF-receptor, scFV-antiNIP, GGGSflexlinker, the trans-membrane region of the Erbb1/EGF-receptor and the N-terminal part of the split-fluorophor YFP. | ||
+ | Part was derived by cloning of BBa_K157001, BBa_K157003, BBa_K157013, BBa_K157002, BBa_K157008. | ||
+ | For further information see the description of the parts mentioned above. | ||
<br> | <br> | ||
'''AASequence:'''<br> | '''AASequence:'''<br> | ||
'''gene sequence:'''<br><br> | '''gene sequence:'''<br><br> | ||
- | * | + | *'''SP-AntiNIP-GGGSlinker-Tm-Fluolinker-cCFP''' |
'''Part Name:'''<br> | '''Part Name:'''<br> | ||
+ | [http://partsregistry.org/wiki/index.php?title=Part:BBa_K157028 BBa_K157028] | ||
'''Description:'''<br> | '''Description:'''<br> | ||
+ | Part consists of the signal peptide of Erbb1/EGF-receptor, scFV-antiNIP, GGGSflexlinker, the trans-membrane region of the Erbb1/EGF-receptor and the C-terminal part of the split fluorophor YFP with a fluolinker joined to its N-Terminus. | ||
+ | Part was derived by cloning of BBa_K157001, BBa_K157003, BBa_K157013, BBa_K157002, BBa_K157009 and BBa_K157005. | ||
+ | For further information see the description of the parts mentioned above. | ||
<br> | <br> | ||
'''AASequence:'''<br> | '''AASequence:'''<br> | ||
'''gene sequence:'''<br><br> | '''gene sequence:'''<br><br> | ||
- | * | + | *'''SP-AntiNIP-GGGSlinker-Tm-nCFP''' |
'''Part Name:'''<br> | '''Part Name:'''<br> | ||
+ | [http://partsregistry.org/wiki/index.php?title=Part:BBa_K157029 BBa_K157029] | ||
'''Description:'''<br> | '''Description:'''<br> | ||
+ | Part consists of the signal peptide of Erbb1/EGF-receptor, scFV-antiNIP, GGGSflexlinker, the trans-membrane region of the Erbb1/EGF-receptor and intracellular the N-terminal part of the split fluorophor YFP. Part was derived by cloning of BBa_K157001, BBa_K157003, BBa_K157013, BBa_K157002, BBa_K157006. | ||
+ | For further information see the description of the parts mentioned above. | ||
<br> | <br> | ||
'''AASequence:'''<br> | '''AASequence:'''<br> |
Revision as of 12:23, 29 October 2008
Parts |
_parts
basic parts
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