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Team:Hawaii/Ligation of pRL1383a Parts
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|---|---|---|---|---|
| Sponsors | Experiments | Milestones | Protocols | |
| Notebook (t) | Meetings (t) |
Contents |
Ligation of Parts
- The BioBrick parts of pRL1383a are to be ligated in a series of experiments.
Methods
- Round 1 of Restriction Digest and Ligation
| Name | size | enzyme | quantity | date of digest |
|---|---|---|---|---|
| rep | 3.3kb | XbaI & PstI | 5ng/ul | 8/11/08 |
| oriV | 415bp | XbaI & PstI | 5ng/ul | 8/11/08 |
| aadA (pRL1383a) | 806bp | XbaI & PstI | 5ng/ul | 8/11/08 |
| aadA (BB) | 806bp | XbaI & PstI | 5ng/ul | 8/11/08 |
| oriT | ~125bp | XbaI & PstI | n/a | 8/27/08 |
| P1 lytic Region | 1.3kb | EcoRI & SpeI | 5ng/ul | 8/11/08 |
| BBa_B0030 | 2094bp | SpeI & PstI | 4ng/ul | 8/11/08 |
| BBa_B0015 | 3318bp | EcoRI & XbaI | 5.7ng/ul | 8/11/08 |
| pSB1A2 | 2094bp | SpeI & PstI | 5.5ng/ul | 8/11/08 |
| BBa_I14032 | 4462bp | n/a | n/a |
- Ligation
| Name |
|---|
| rep(80ng)+B0030(25ng) |
| oriV(20ng)+pSB1A2(50ng) |
| aadA (BB) |
- Transformation
- Verification with Colony PCR
Results
Discussion
- What was learned and how to do future experiments differently.
