"
Team:Hawaii/Notebook/2008-06- 1
From 2008.igem.org
(Difference between revisions)
(New page: {{Team:Hawaii/Header}} == What we did today == '''Grace, Margaret, Norman''' * Autoclaved MgSO<sub>4</sub> * Made DH5-alpha seed stock * Grew DH5-alpha in SOB media at 23C, with shaking...) |
|||
| Line 5: | Line 5: | ||
* Autoclaved MgSO<sub>4</sub> | * Autoclaved MgSO<sub>4</sub> | ||
| - | * Made DH5-alpha seed stock | + | * Made DH5-α seed stock |
| - | * Grew DH5-alpha in SOB media at 23C, with shaking @ 250 rpm (will take ~16 hours) | + | * Grew DH5-α in SOB media at 23C, with shaking @ 250 rpm (will take ~16 hours) |
* Designed PCR protocol | * Designed PCR protocol | ||
:* Practiced using Primer 3 | :* Practiced using Primer 3 | ||
Latest revision as of 04:39, 3 June 2008
| Projects | Events | Resources | ||
|---|---|---|---|---|
| Sponsors | Experiments | Milestones | Protocols | |
| Notebook (t) | Meetings (t) |
What we did today
Grace, Margaret, Norman
- Autoclaved MgSO4
- Made DH5-α seed stock
- Grew DH5-α in SOB media at 23C, with shaking @ 250 rpm (will take ~16 hours)
- Designed PCR protocol
- Practiced using Primer 3
Discussion
[http://manoa.hawaii.edu/ 
][http://manoa.hawaii.edu/ovcrge/ 
][http://www.ctahr.hawaii.edu 
]
