Revision as of 22:05, 16 July 2008 by MargaretRuzicka (Talk | contribs)

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Things we did today

Wetlab work

Margaret

pSB3K3 was restreaked yesterday, and today I am growing them up in LB media in preparation for a plasmid prep.

checked transformants from yesterday. The controls came out as expected (positive: lawn, negative:lawn(oops, i didn't add selection to the plate)), neither pSB3K3, pSB1A2, nor pSB1AK3 transformed.

I extracted pSB1A2 & pSB1AK3 today with 7uL TE. All 7uL was used to transform DB3.1 cells again.

Last night, I digested the oriT ligation with PstI, left it overnight. This morning, I stopped the digestion, precipitated the DNA and stored it in TE in the -20C freezer. The protocol can be found here.

name of person/people who performed the task

Summary of task and what was done. Link to experiment for detailed notes if necessary.
e.g. read through papers, worked on proposal, etc.

History is the only laboratory we have in which to test the consequences of thought. - Étienne Gilson