Revision as of 04:00, 22 July 2008

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Things we did today

Wetlab work

Colony PCR

Margaret

Colony PCR of pSB1A2, pSB1A3, pSB3K3

Lane 1 contains the 2-log ladder, lane 2: pSB1A3's ccdB insert, lane3: pSB1A2's ccdB insert, lane 4: pSB3K3's ccdB insert, lane 5: positive control, lane 6: negative control, lane 7: pSMC121.

PCR amplification of pRL1383a parts

continued from Saturday, ran a gel

Drylab Work

Name of Task

name of person/people who performed the task

Summary of task and what was done. Link to experiment for detailed notes if necessary.
e.g. read through papers, worked on proposal, etc.

Discussion

Quote of the Day

History is the only laboratory we have in which to test the consequences of thought. - Étienne Gilson

[http://manoa.hawaii.edu/ ][http://manoa.hawaii.edu/ovcrge/ ][http://www.ctahr.hawaii.edu ]

@@ Line 7: / Line 7: @@
 :* Colony PCR of pSB1A2, pSB1A3, pSB3K3
+[[Image:7_21_08_annotated.jpg|right|thumb|300px|Lane 1 contains the 2-log ladder, lane 2: pSB1A3's ccdB insert, lane3: pSB1A2's ccdB insert, lane 4: pSB3K3's ccdB insert, lane 5: positive control, lane 6: negative control, lane 7: pSMC121.]]
 ===PCR amplification of pRL1383a parts===
-[[Image:mob_re_omega.jpg|right|thumb|300px|Lane 1 and 12 is the tridye 2-log ladder, 2,3,4, &5 are the mob/rep region, the omega interposon, the negative and positive control, respectively amplified with Green Taq. Lanes 6 and 7 are the mob/rep and omega amplified with accuzyme. Lanes 8,9,10,&11 are the mob/rep region, the omega interposon, the negative and positive control, respectively amplified with velocity taq.]]
 :*continued from [[Team:Hawaii/Notebook/2008-07-19|Saturday]], ran a gel

Team:Hawaii/Notebook/2008-07-21

From 2008.igem.org